Albuquerque, Inês S.Jeremias, Hélia F.Chaves-Ferreira, MiguelMatak-Vinkovic, DijanaBoutureira, OmarRomão, Carlos C.Bernardes, Gonçalo J. L.2022-05-262022-05-262015Chem Commun (Camb). 2015 Mar 7;51(19):3993-39961359-7345http://hdl.handle.net/10451/53200This journal is © The Royal Society of Chemistry 2015. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.We report the design and synthesis of an aquacarbonyl Ru(II) dication cis-[Ru(CO)2(H2O)4](2+) reagent for histidine (His)-selective metallation of interleukin (IL)-8 at site 33. The artificial, non-toxic interleukin (IL)-8-Ru(II)(CO)2 metalloprotein retained IL-8-dependent neutrophil chemotactic activity and was shown to spontaneously release CO in live cells.engjournal article10.1039/C4CC10204E1364-548X