Abreu, Marta Maria NogueiraMartins, Margarida MoldãoAleixo, Cristina Maria MarquesRufino, Joana da Silva2015-03-202015-03-202014Rufino, J.S. - Tratamentos pós-colheita não químicos: biocontrolo e calor na conservação de pera Rocha inteira e na aptidão para processamento. Lisboa: ISA, 2014, 99 p.http://hdl.handle.net/10400.5/8264Mestrado em Engenharia Alimentar - Instituto Superior de AgronomiaIntegration of biocontrol and heat treatment (40 C / 40 min) as non-chemical alternatives to Rocha pear postharvest storage and respective effects to pulp production were evaluated. From native microflora characterization, counts up to 5x104cfu.g-1 in yeasts and moulds (Y&M) and aerobic microorganisms at 30 °C were found and Cladosporium sp. was identified as the dominant pathogen in Rocha pear at harvest. The lack of effects from the commercial antagonist Blossom Protect™™ (Aureobasidium pullulans,108 cfu.g-1) against Cladosporium sp. during tested storage conditions (7 °C, 60 days) were countered by in vitro results where inhibitions up to 80% were found with 108cfu.g-1 viable cells. Heat treatment application proved to effectively control (100%) postharvest rot caused by Penicillium expansum, found to be the dominant fungi under simulated postharvest conditions. Treatment combination did not introduced additional effects to the found efficiency. To Rocha pear pulp production, the introduction of the heat treatment as a raw material decontamination operation demonstrated to significantly reduce initial Y&M contamination. These results suggest that the heat treatment is an efficient eco-friendly alternative to the widespread use of sodium hypochlorite. However, the heat treatment had no inhibitory effect over excessive colour changes occurring after cut and smash operationsporRocha pearpostharvestbiocontrolheat treatmentCladosporiumqualitymaster thesis203700481