Matos, Maria J.Brown, LibbyBernardim, BarbaraGuerreiro, AnaJiménez-Osés, GonzaloBernardes, Gonçalo J. L.2021-05-272021-05-272020Bioorg Med Chem. 2020 Nov 15;28(22):1157830968-0896http://hdl.handle.net/10451/48213© 2020 Elsevier Ltd. All rights reserved.Methods that allow for chemical site-selective dual protein modification are scarce. Here, we provide proof-of-concept for the orthogonality and compatibility of a method for regioselective lysine modification with strategies for protein modification at cysteine and genetically encoded ketone-tagged amino acids. This sequential, orthogonal approach was applied to albumin and a therapeutic antibody to create functional dual site-selectively labelled proteins.engAlbuminAntibodiesBioorthogonal labellingLysine modificationProtein modificationSequential dual site-selective protein labelling enabled by lysine modificationjournal article10.1016/j.bmc.2020.1157831464-3391