Paiva, Jorge PintoAlmeida, Maria Helena RibeiroBarrela, Ricardo Joel Martins Bernardino Marques2012-03-262012-03-262011Barrela, Ricardo Joel Martins Bernardino Marques - Identificação e sequenciação de genes da via da lenhificação em bibliotecas BAC de Eucalyptus globulus. Lisboa: ISA, 2011http://hdl.handle.net/10400.5/4188Mestrado em Engenharia Florestal e dos Recursos Naturais - Instituto Superior de AgronomiaIn this work we characterized two libraries containing BAC clones - EGC_Ba and EGC_Bb - obtained from fragments of genomic DNA of Eucalyptus globulus digested with HindIII and EcoRI respectively. The estimated genome coverage for the two libraries was 16X. Both libraries had inserts with average size of 115kb. Assessment of contamination by extranuclear genome was performed by hybridization of high-density colony filters, ensuring a low rate of contamination. The probability associated with the attempt to find specific sequences in the libraries was calculated at a value greater than 0.99. The identification of several genes involved in lignin biosynthetic pathway, transcription factors and peripheral regions of interest via PCR was performed using an innovative scheme of three-dimensional pools. Of the clones identified, thirteen were chosen for sequencing. The analysis of the sequences obtained allowed identifying the reduced presence of repetitive elements and microsatellites. The identified clones can be used in future researches involving the isolation of genes of QTL and genomic sequence comparison with other species of Eucalyptus.porEucalyptus globulusBAC librarylignin biosynthesis pathway3 D poolmaster thesis