Fernandes, João C.Schemitt, ElizangelaSilva, JulianaMarroni, Norma P.Lima, AnaFerreira, Ricardo Boavida2021-11-042021-11-042021Fernandes, J.C.; Schemitt, E.G.; Da Silva, J.; Marroni, N.P.; Lima, A.; Ferreira, R.B. Combination of Trans-Resveratrol and E-Viniferin Induces a Hepatoprotective Effect in Rats with Severe Acute Liver Failure via Reduction of Oxidative Stress and MMP-9 Expression. Nutrients 2021, 13, 3677http://hdl.handle.net/10400.5/22494Stilbenes are a major grapevine class of phenolic compounds, known for their biological activities, including anti-inflammatory and antioxidant, but never studied in combination. We aimed to evaluate the effect of trans-resveratrol + "-viniferin as an antioxidant mixture and its role in inflammatory development an in vivo model of severe acute liver failure induced with TAA. Trans-resveratrol + trans-"-viniferin (5 mg/kg each) was administered to Wistar rats. Resveratrol + "- viniferin significantly decreased TBARS and SOD activity and restored CAT and GST activities in the treated group. This stilbene combination reduced the expression of TNF , iNOS, and COX-2, and inhibited MMP-9. The combination of resveratrol + "-viniferin had a hepatoprotective effect, reducing DNA damage, exhibiting a protective role on the antioxidant pathway by altering SOD, CAT, and GST activities; by downregulating TNF , COX-2, and iNOS; and upregulating IL-10. Our results suggested that adding viniferin to resveratrol may be more effective in hepatoprotection than resveratrol alone, opening a new perspective on using this stilbene combination in functional dietsenginflammationliver failureMMP-9oxidative stressresveratrolstilbenesthioacetamideE-viniferinjournal article