Martins, Margarida MoldãoGonçalves, José Carlos Dias DuarteAlves, Vitor Manuel DelgadoCoelho, Maria Teresa Pita Pegado Gonçalves Rodrigues2015-09-252015-09-252015Coelho, M.T.P.P.G.R. - Estudos de propagação in vitro, caracterização e valorização de carqueja (Pterospartum tridentatum (L.) Willk). Lisboa: ISA, 2015, 287 p.http://hdl.handle.net/10400.5/9271Doutoramento em Engenharia Alimentar - Instituto Superior de AgronomiaThe aim of this work was to develop methodologies for micropropagation and extraction of bioactive compounds from both wild gorse (Pterospartum tridentatum L.) and micropropagated gorse explants, as a means of valorisation of this species. Micropropagation was very effective on large scale propagation, having multiplication rates ranging from 1.6 to 43.9; number of shoots ranging from 1.5 to 10.4 and rooting above 96.7%. The extraction yield of aqueous extraction varied from 10.5 to 19.4 and 12.2 to 30.1% (w/w) for wild gorse and micropropagated gorse explants, respectively. Aqueous extracts showed high levels of total phenols, from 203.4 to 369.2 and 104.1 to 121.3 mg gallic acid eq g-1 ms, and FRAP antioxidant activity: 256-856 and 160-260 mmolFe2+ 100g-1 ms; DPPH: 1433-1551 and 1503-1509 mM Trolox 100g-1 ms for wild gorse and micropropagated gorse explants, respectively. Extracts showed no antifungal activity. However, these extracts showed inhibition of Bacillus subtilis, Pseudomonas aeruginosa and Staphylococcus aureus. Chitosan bioactive coatings with gorse extracts improved cherry postharvest preservation ability. The results allow topropose these methodologies to obtain extracts with bioactive properties from micropropagated material, without depleting natural resourcesporPterospartum tridentatumethnobotanymicropropagationaqueous extractsbiological activitydoctoral thesis101483627