The challenge of peptide proteolytic stability studies: scarce data, difficult readability, and the need for harmonization

Cavaco, MarcoAndreu, DavidCastanho, Miguel A. R. B.2023-05-102023-05-102020Angew. Chem. 2021, 133, 1710-17120044-82491521-3757http://hdl.handle.net/10451/57398© 2020 Wiley-VCH GmbHProteolytic stability assessment is increasingly viewed as a fundamental component of peptide characterization, arguably of comparable importance as efficacy and toxicity data. A literature survey over the last decade reveals steady growth in the stability information available. However, it also uncovers two significant problems that hinder proper data comparison: 1) the use of different stability assays, and 2) the differences in how stability information is reported. In this Viewpoint, we present results from a database meta-analysis as well as concerns about the stability assessments published so far. We also suggest guidelines for a proper discussion between experts in the field on how to improve data readability so that peptide stability, an often-missing parameter in older literature, is adequately reported to take maximum advantage of it.engDegradationPeptidesProteolysisStabilityThe challenge of peptide proteolytic stability studies: scarce data, difficult readability, and the need for harmonizationjournal article10.1002/ange.20200637210.1002/anie.202006372