Cortes, Rui ManuelVarandas, SimoneHughes, Samantha JaneFerreira, Maria Teresa2013-02-122013-02-122008"Limnetica". ISSN 0213-8409. 27 (1) (2008) 39-560213-8409http://hdl.handle.net/10400.5/5142The River Habitat Survey (RHS) is a widespread technique used to characterize the habitats at the fluvial sector level and to quantify the magnitude of physical disturbances. In this study we tested the ability of this method to discriminate sites along a gradient of perturbation and to analyse its relation with typological and environmental features. At the same time, a biological validation of RHS parameters was carried out by crossing the descriptors with three different aquatic communities (benthic macroinvertebrates, fish, and macrophytes). The Portuguese sector of the Douro basin was the area selected for this purposes. Ordination and classification techniques were used to achieve the objectives mentioned, after a reduction of the variables as a function of their relationships and according to their affinity. The multivariate analysis revealed that the variables associated with the river corridor were more appropriate to differentiate the sampling stations along the gradient of disturbance. However, these variables, together with the ones describing the river conditions (together with the RHS derived indices) were strongly dependent on the typological characteristics. This work shows an adequate strategy for the characterization of the fluvial habitat with biological data to obtain an integrated ecological vision.engRHSbiological characterizationphysical characteristicsintegral ecological assessmentjournal article