Mota, Mariana GomesOliveira, Cristina Maria Moniz SimõesLopes, João Pedro da Ponte Soares2012-03-212012-03-212011Lopes, João Pedro da Ponte Soares - Polinização em oliveira cvs. Galega e Cobrançosa: avaliação do vingamento em ensaios de polinização controlada e do efeito da aplicação de um bioestimulante. Lisboa: ISA, 2011http://hdl.handle.net/10400.5/4122Mestrado em Engenharia Agronómica - Instituto Superior de AgronomiaThree pollination methods were tested in olive cultivars Galega and Cobrançosa: auto-pollination, free pollination and cross-pollination (using pollen from cultivar Maçanilha). At the same time the application of a biostimulant based in seaweed was studied. The field trial was conducted in an olive orchard near Santarém. Due to heavy winds and rain it was not possible to carry out the cross-pollination experiments and the autopollination assay for „Cobrançosa‟. Although the cross-pollination experiments were not done, the quality of the Maçanilha pollen was tested and showed good quality. It was possible to establish a fast test to analyse the pollen viability in the farm. Biostimulant action was analysed in terms of fruit set and activity of enzymes involved in nitrogen metabolism (NR and GS) in leaves and in reproductive organs. Results showed that the biostimulant significantly improved fruit set in „Galega‟ and the enzymatic activity in reproductive organs of „Cobrançosa‟. It was concluded that the biostimulant effects on enzymatic activity was more expressive in the reproductive organs and in trees grown under suboptimal conditions.porOlea europaeacontrolled pollinationbiostimulantfruit-setnitrate redutaseglutamine synthetasemaster thesis