Santos, Andrea Martins dos2014-03-272014-03-272012J Fluoresc (2012) 22:151–1541053-0509http://dx.doi.org/10.1007/s10895-011-0941-0http://hdl.handle.net/10451/10789© Springer Science+Business Media, LLC 2011.The emission behaviour of Aequorea green fluorescent protein (A-GFP) chromophore, in both neutral (N) and anionic (A) form, was studied in the temperature range from 20 °C to 75 °C and at pH=7. Excitation wavelengths of 399 nm and 476 nm were applied to probe the N and A forms environment, respectively. Both forms exhibit distinct fluorescence patterns at high temperature values. The emission quenching rate, following a temperature increase, is higher for the chromophore N form as a result of the hydrogen bond network weakening. The chromophore anionic form emission maximum is red shifted, upon temperature increase, due to a charge transfer process occurring after A form excitation.engGreen fluorescent proteinChromophoreFluorescenceTemperaturejournal article