Carvalho, Ricardo CruzPaço, TeresaBranquinho, CristinaSilva, Jorge Marques2021-07-162021-07-162020Water 2020, 12, 1748http://hdl.handle.net/10400.5/21630Green roofs have been more thoroughly investigated in the last few years due to the potential benefits they o er to ecosystems in urban areas (e.g., carbon sequestration, particle retention, heat island e ect attenuation). However, current climate change models predict an increase in desertification, with an increase in temperature and decrease in rainfall, which means there is an increasing demand for green roofs with lower water consumption. Vegetation with very little water requirements, such as desiccation-tolerant mosses, has shown a potential to complement or substitute for vascular species, increasing the sustainability of lower water use in green roofs. In this study, we use chlorophyll a fluorescence imaging to screen for bryophytes with adequate physiology to be used in green roofs placed in at-risk areas with prolonged drought episodes. Apart from Hypnum cupressiforme, all selected species presented a high potential for use in those conditions, particularly Didymodon fallax, Grimmia lisae, Pleurochaete squarrosa, and Targionia hypophylla. Chlorophyll a fluorescence imaging technology proved to be a simple and non-invasive tool for a fast screening of these poikilohydric organisms, to be used in future studies of bryophyte biology, but more importantly in the green roof industryenggreen roofsmosseschlorophyll a fluorescencewater usesustainabilityMediterraneanbiological soil crustsjournal article10.3390/w12061748