Roseiro, Luísa Cristina PereiraAlmeida, João Augusto MarquesMourato, Miguel BarbosaVieira, Bárbara Daniela Tavares Neiva2012-03-262012-03-262011Vieira, Bárbara Daniela Tavares Neiva - Determinação simultânea das vitaminas do complexo B em carne de bovino por HPLC. Lisboa: ISA, 2011http://hdl.handle.net/10400.5/4185Mestrado em Engenharia Alimentar - Instituto Superior de AgronomiaThe main objective of this study was the simultaneous determination of vitamins B1, B2, B3, B5, B6, B9 and B12 in beef, using a simple and rapid HPLC method. Several tests were conducted in order to optimize the extraction technique and the chromatographic conditions for an effective determination of those vitamins. After the optimization of the extraction and separation/quantification procedures it was necessary to establish a relation between the content of the vitamin that exists in the meat and the characteristics of the animal in study. The results show that the extraction method is effective for vitamins B1, B2 and B6, as the obtained recovery rates fall within the established limits (61.3%, 55,5%, and 76,4%, respectively). Regarding linearity, the correlation coefficients obtained for all cases show that the method has a high correlation. The study also showed that the average levels of vitamins are influenced by several factors. Through this study, we can conclude that the simultaneous determination of vitamins of the B complex is possible, using a fast and simple HPLC method.pormeatwater soluble vitaminsextractionRP-HPLCmaster thesis