Leites, ElviraMorais, Vanessa A.2025-05-302025-05-302024STAR Protoc. 2024 Mar 15;5(1):102804http://hdl.handle.net/10400.5/101153© 2023 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Studying the intrinsic properties of microglia, astrocytes, and neurons is essential to our understanding of brain function. Here, we present a protocol to isolate and culture these neural cells from the same mouse brain. Using immunocapture magnetic beads, we describe steps for dissociating, cleaning, and sequentially separating brains from 9-day-old mice into microglia, astrocytes, and neurons. Following these detailed procedures for seeding and culturing of isolated cells, we can address critical questions related to brain function.engCell cultureCell isolationCell-based AssaysMicroscopyNeurosciencejournal article10.1016/j.xpro.2023.1028042666-1667