Ferreira, Maria Adélia SantosSousa, Isabel Maria Nunes dePintado, Cristina Maria Baptista Santos2010-01-082010-01-082009http://hdl.handle.net/10400.5/1662Doutoramento em Engenharia Agro-industrial - Instituto Superior de AgronomiaStrains of Listeria monocytogenes, L.innocua and L. seeligeri, isolated from two regions producing ewe's cheese (Castelo Branco and Tolosa) were characterized. Using molecular typage (AFLP, PFGE and molecular serotyping) it was possible to do the association between a case of asymptomatic ovine listeriosis and the contamination of cheese made with raw milk and the environment, as the same molecular type was revealed (AFLP IV-1, PFGE 11 and molecular serogroup 4. The molecular characterization of 185 isolates permitted to study the routes of contamination and the persistence of L. monocytogenes with cheese processing plant.porListeria monocytogenescheeseantimicrobial coatingsnisinmolecular typageovine listeriosisqueijo de ovelhalisteriose ovinanisinatipagem moleculardoctoral thesis