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http://hdl.handle.net/10451/21317| Título: | Plasmodium chabaudi Expression of active recombinant chabaupain-1 and localization studies in Anopheles sp. |
| Autor: | Caldeira, Rubina L. Goncalves, Lidia M. D. Martins, Tiago M. Silveira, Henrique Novo, Carlos do Rosario, Virgilio Domingos, Ana |
| Palavras-chave: | Parasitology |
| Data: | 2009 |
| Editora: | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| Citação: | EXPERIMENTAL PARASITOLOGY. - Vol. 122, n. 2 (JUN 2009), p. 97-105 |
| Resumo: | Plasmodium cysteine proteases have been shown to be immunogenic and are being used as malaria potential serodiagnostic markers and vaccine targets. Genes encoding two Plasmodium chabaudi cysteine proteases chabaupain-1 (CP-1) and chabaupain-2 (CP-2) were identified and further expressed in Escherichia coli. Solubilisation of recombinant CP-1 and CP-2 was achieved by decreasing the temperature of induction. Anopheles gambiae tissues infected with Plasmodium were analyzed by Western blotting using the anti-CP-1 antibody showing that CP-1 is only present in the A. gambiae midguts being absent from other infected mosquito biological material. Anti-CP-1 anti-serum recognized a 30 kDa band in P. chabaudi, Plasmodium berghei and Plasmodium yoelii lysates but does not recognize the recombinant CP-2 extracts suggesting high antibody specificity. (c) 2009 Elsevier Inc. All rights reserved.. - Portuguese Foundation for Science and Technology (FCT) [POCTI/SAU-ESP/57696/2004]. - This research was funded by the Portuguese Foundation for Science and Technology (FCT) under the project POCTI/SAU-ESP/57696/2004. |
| URI: | http://hdl.handle.net/10451/21317 |
| DOI: | http://dx.doi.org/10.1016/j.exppara.2009.03.003 |
| ISSN: | 0014-4894 |
| Aparece nas colecções: | FF - Produção Científica 2000-2009 |
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