| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 1.74 MB | Adobe PDF |
Authors
Abstract(s)
As doenças periodontais, nomeadamente gengivite e periodontite, estão entre as condições mais prevalentes a nível global, sendo caracterizadas por serem patologias não comunicáveis, fruto de uma resposta inflamatória crónica, que afetam a cavidade oral. Recentemente, a progressão de periodontite tem sido associada a diversas repercussões sistémicas, incluindo infertilidade feminina e resultados adversos na gravidez, contudo, os mecanismos associados mantêm-se desconhecidos. Desta forma, este projeto tem como objetivo desenvolver um modelo animal de periodontite, em ratos, para avaliar a influência desta doença na fertilidade feminina, elucidando os mecanismos envolvidos. Assim sendo, foram desenvolvidos dois modelos animais de periodontite diferentes, de forma a perceber qual o mais adequado. O primeiro modelo animal foi desenvolvido através de administrações gengivais de lipopolissacarídeos, obtidos através de Porphyromonas gingivalis, e o segundo pela aplicação e manutenção de ligaduras nos molares superiores. Os ratos foram avaliados a nível de sinais clínicos e marcadores hematológicos, bioquímicos, endócrinos e imunológicos. Adicionalmente, foi feita uma avaliação macroscópica e microscópica na gengiva dos animais, bem como do sistema reprodutor. Considerando os resultados obtidos, o modelo animal de periodontite induzido através de ligaduras foi o mais adequado, tendo em conta o desenvolvimento de uma inflamação crónica no periodonto, bem como manifestações sistémicas que poderão estar associadas a infertilidade feminina. De facto, foi observada uma redução pronunciada na expressão de estradiol, triiodotironina e tiroxina, aliado a um aumento nos níveis de proteína c-reativa, imunoglobulina G e lactato desidrogenase. Concluindo, o objetivo principal para este projeto foi atingido com sucesso, tendo em conta o desenvolvimento de um modelo animal de periodontite, em ratos, adequado para estudar a sua influência na fertilidade feminina. Adicionalmente, os resultados obtidos poderão ter um papel fulcral no futuro, visto que é a primeira vez que são demonstrados os potenciais mecanismos associados à correlação entre periodontite e infertilidade feminina.
Periodontal diseases, namely gingivitis and periodontitis, are among the most prevalent conditions worldwide, being characterized as non-communicable and chronic inflammatory disorders that affect the oral cavity. Recently, the progression of periodontitis has been associated with several systemic repercussions, including female infertility and adverse pregnancy outcomes, however, the underlying mechanism(s) remains unclear. Therefore, this project aims to develop a rat model of periodontitis to evaluate the influence of this disease on female fertility, elucidating the specific mechanism(s) involved. Indeed, it was developed two different rat models of periodontitis to understand the most suitable one. The first animal model was developed through gingival administrations of lipopolysaccharides, obtained from Porphyromonas gingivalis, and the second one with the application and maintenance of ligatures, in the upper molars. The rats were assessed in terms of clinical signs, hematological, biochemical, endocrine, and immunological markers. Additionally, it was performed a macroscopical and microscopical evaluation in the gingiva of the animals, as well as in the reproductive system. Considering the results, the ligature-induced rat model of periodontitis was the most reliable since it was possible to observe an exacerbated inflammatory response in the periodontium, along with significant systemic manifestations that can be related to female infertility. In fact, it was noticed a pronounced reduction in the expression of estradiol, triiodothyronine, and thyroxine, along with the increase in the levels of c-reactive protein, immunoglobulin G and lactate dehydrogenase. Concluding, the major goal proposed for this project was successfully attained, considering the development of a reliable rat model of periodontitis to study its influence in female fertility. Additionally, the results obtained may have a pivotal role in the future since it is the first time that the potential biological mechanisms associated with the relationship between periodontitis and female infertility are described.
Periodontal diseases, namely gingivitis and periodontitis, are among the most prevalent conditions worldwide, being characterized as non-communicable and chronic inflammatory disorders that affect the oral cavity. Recently, the progression of periodontitis has been associated with several systemic repercussions, including female infertility and adverse pregnancy outcomes, however, the underlying mechanism(s) remains unclear. Therefore, this project aims to develop a rat model of periodontitis to evaluate the influence of this disease on female fertility, elucidating the specific mechanism(s) involved. Indeed, it was developed two different rat models of periodontitis to understand the most suitable one. The first animal model was developed through gingival administrations of lipopolysaccharides, obtained from Porphyromonas gingivalis, and the second one with the application and maintenance of ligatures, in the upper molars. The rats were assessed in terms of clinical signs, hematological, biochemical, endocrine, and immunological markers. Additionally, it was performed a macroscopical and microscopical evaluation in the gingiva of the animals, as well as in the reproductive system. Considering the results, the ligature-induced rat model of periodontitis was the most reliable since it was possible to observe an exacerbated inflammatory response in the periodontium, along with significant systemic manifestations that can be related to female infertility. In fact, it was noticed a pronounced reduction in the expression of estradiol, triiodothyronine, and thyroxine, along with the increase in the levels of c-reactive protein, immunoglobulin G and lactate dehydrogenase. Concluding, the major goal proposed for this project was successfully attained, considering the development of a reliable rat model of periodontitis to study its influence in female fertility. Additionally, the results obtained may have a pivotal role in the future since it is the first time that the potential biological mechanisms associated with the relationship between periodontitis and female infertility are described.
Description
Tese de mestrado, Ciências Biofarmacêuticas, 2023, Universidade de Lisboa, Faculdade de Farmácia.
Keywords
Periodontitis Inflammation Female infertility Animal models Rodents Teses de mestrado - 2023