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Genome-wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factors

2006Artigo científico Acesso aberto
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dc.contributor.authorGama-Carvalho, Margarida
dc.contributor.authorBarbosa-Morais, Nuno
dc.contributor.authorBrodsky, Alexander S.
dc.contributor.authorSilver, Pamela A.
dc.contributor.authorCarmo-Fonseca, Maria
dc.date.accessioned2021-05-10T16:44:09Z
dc.date.available2021-05-10T16:44:09Z
dc.date.issued2006
dc.description© 2006 Gama-Carvalho et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.pt_PT
dc.description.abstractBackground: Pre-mRNA splicing is an essential step in gene expression that occurs co-transcriptionally in the cell nucleus, involving a large number of RNA binding protein splicing factors, in addition to core spliceosome components. Several of these proteins are required for the recognition of intronic sequence elements, transiently associating with the primary transcript during splicing. Some protein splicing factors, such as the U2 small nuclear RNP auxiliary factor (U2AF), are known to be exported to the cytoplasm, despite being implicated solely in nuclear functions. This observation raises the question of whether U2AF associates with mature mRNA-ribonucleoprotein particles in transit to the cytoplasm, participating in additional cellular functions. Results: Here we report the identification of RNAs immunoprecipitated by a monoclonal antibody specific for the U2AF 65 kDa subunit (U2AF65) and demonstrate its association with spliced mRNAs. For comparison, we analyzed mRNAs associated with the polypyrimidine tract binding protein (PTB), a splicing factor that also binds to intronic pyrimidine-rich sequences but additionally participates in mRNA localization, stability, and translation. Our results show that 10% of cellular mRNAs expressed in HeLa cells associate differentially with U2AF65 and PTB. Among U2AF65-associated mRNAs there is a predominance of transcription factors and cell cycle regulators, whereas PTB-associated transcripts are enriched in mRNA species that encode proteins implicated in intracellular transport, vesicle trafficking, and apoptosis. Conclusion: Our results show that U2AF65 associates with specific subsets of spliced mRNAs, strongly suggesting that it is involved in novel cellular functions in addition to splicing.pt_PT
dc.description.sponsorshipThis work was supported by grants from Fundação para a Ciência e Tecnologia (FCT), Portugal in association with the European Science Foundation (ESF) under the EUROCORES Programme EuroDYNA. ASB was funded by a K22 NHGRI award. MGC was funded by a short-term Human Frontiers Science Program Fellowship. NLBM was funded by FCT (Fellowship SFRH/BD/2914/2000).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGenome Biol. 2006;7(11):R113pt_PT
dc.identifier.doi10.1186/gb-2006-7-11-r113pt_PT
dc.identifier.eissn1465-6906
dc.identifier.issn1474-760X
dc.identifier.urihttp://hdl.handle.net/10451/47732
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Naturept_PT
dc.relation.publisherversionhttps://genomebiology.biomedcentral.com/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleGenome-wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factorspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberSFRH/BD/2914/2000
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F2914%2F2000/PT
oaire.citation.issue11pt_PT
oaire.citation.startPageR113pt_PT
oaire.citation.titleGenome Biologypt_PT
oaire.citation.volume7pt_PT
oaire.fundingStreamSFRH
person.familyNameda Gama Carvalho
person.familyNameBARBOSA MORAIS
person.familyNameCarmo-Fonseca
person.givenNameMargarida
person.givenNameNUNO LUÍS
person.givenNameMaria
person.identifier111193
person.identifierI-2743-2013
person.identifier.ciencia-id4710-D955-1EAB
person.identifier.ciencia-id5C19-CA77-A74D
person.identifier.ciencia-idB31F-0435-0753
person.identifier.orcid0000-0002-0365-6916
person.identifier.orcid0000-0002-1215-0538
person.identifier.orcid0000-0002-3402-7143
person.identifier.ridE-6180-2012
person.identifier.scopus-author-id6602645433
person.identifier.scopus-author-id6507555084
person.identifier.scopus-author-id7007128195
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication32f177ba-0062-49bc-a57e-0b3366809580
relation.isAuthorOfPublication7ebfc1e4-56dd-40a2-bb4c-5086cd039ee2
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