Publication
25-hydroxycholesterol effect on membrane structure and mechanical properties
| dc.contributor.author | Domingues, Marco | |
| dc.contributor.author | Gomes, Bárbara | |
| dc.contributor.author | Hollmann, Axel | |
| dc.contributor.author | Santos, Nuno C. | |
| dc.date.accessioned | 2022-09-06T11:29:37Z | |
| dc.date.available | 2022-09-06T11:29:37Z | |
| dc.date.issued | 2021 | |
| dc.description | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | pt_PT |
| dc.description.abstract | Cholesterol is responsible for the plasticity of plasma membranes and is involved in physiological and pathophysiological responses. Cholesterol homeostasis is regulated by oxysterols, such as 25-hydroxycholesterol. The presence of 25-hydroxycholesterol at the membrane level has been shown to interfere with several viruses' entry into their target cells. We used atomic force microscopy to assess the effect of 25-hydroxycholesterol on different properties of supported lipid bilayers with controlled lipid compositions. In particular, we showed that 25-hydroxycholesterol inhibits the lipid-condensing effects of cholesterol, rendering the bilayers less rigid. This study indicates that the inclusion of 25-hydroxycholesterol in plasma membranes or the conversion of part of their cholesterol content into 25-hydroxycholesterol leads to morphological alterations of the sphingomyelin (SM)-enriched domains and promotes lipid packing inhomogeneities. These changes culminate in membrane stiffness variations. | pt_PT |
| dc.description.sponsorship | This work was supported by Fundação para a Ciência e a Tecnologia—Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal) project PTDC/BBB-BQB/3494/2014, as well as by the FCT-MCTES program of “stimulus of scientific employment” CEECIND/02961/2017 to MMD. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Int J Mol Sci. 2021 Mar 4;22(5):2574 | pt_PT |
| dc.identifier.doi | 10.3390/ijms22052574 | pt_PT |
| dc.identifier.eissn | 1422-0067 | |
| dc.identifier.issn | 1661-6596 | |
| dc.identifier.uri | http://hdl.handle.net/10451/54333 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation | Broad-spectrum antiviral peptides against respiratory viruses | |
| dc.relation | Not Available | |
| dc.relation.publisherversion | https://www.mdpi.com/journal/ijms | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | 25-hydroxycholesterol | pt_PT |
| dc.subject | Atomic force microscopy | pt_PT |
| dc.subject | Cholesterol | pt_PT |
| dc.subject | Force spectroscopy | pt_PT |
| dc.subject | Supported lipid bilayers | pt_PT |
| dc.title | 25-hydroxycholesterol effect on membrane structure and mechanical properties | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Broad-spectrum antiviral peptides against respiratory viruses | |
| oaire.awardTitle | Not Available | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-BQB%2F3494%2F2014/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F02961%2F2017%2FCP1396%2FCT0008/PT | |
| oaire.citation.issue | 5 | pt_PT |
| oaire.citation.title | International Journal of Molecular Sciences | pt_PT |
| oaire.citation.volume | 22 | pt_PT |
| oaire.fundingStream | 3599-PPCDT | |
| oaire.fundingStream | CEEC IND 2017 | |
| person.familyName | Domingues | |
| person.familyName | Gomes | |
| person.familyName | Hollmann | |
| person.familyName | Santos | |
| person.givenName | Marco | |
| person.givenName | Bárbara | |
| person.givenName | Axel | |
| person.givenName | Nuno | |
| person.identifier | 198825 | |
| person.identifier.ciencia-id | E317-92F8-84C6 | |
| person.identifier.ciencia-id | 5213-15BD-BF32 | |
| person.identifier.ciencia-id | 9E16-D9F8-1807 | |
| person.identifier.ciencia-id | CD13-5E3A-A3C5 | |
| person.identifier.orcid | 0000-0003-1502-1421 | |
| person.identifier.orcid | 0000-0003-4506-182X | |
| person.identifier.orcid | 0000-0002-8060-2997 | |
| person.identifier.orcid | 0000-0002-0580-0475 | |
| person.identifier.rid | A-5933-2018 | |
| person.identifier.rid | N-7248-2013 | |
| person.identifier.scopus-author-id | 15848341400 | |
| person.identifier.scopus-author-id | 55940818300 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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