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New nanoparticles for topical ocular delivery of erythropoietin

2020-02Artigo científico Acesso restrito
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dc.contributor.authorSilva, Beatriz
dc.contributor.authorMarto, Joana
dc.contributor.authorBraz, Berta São
dc.contributor.authorDelgado, Esmeralda
dc.contributor.authorAlmeida, António José
dc.contributor.authorGonçalves, Lídia
dc.date.accessioned2024-01-22T17:08:53Z
dc.date.available2024-01-22T17:08:53Z
dc.date.issued2020-02
dc.date.updated2023-02-27T18:29:10Z
dc.description© 2020 Elsevier B.V. All rights reserved.pt_PT
dc.description.abstractErythropoietin (EPO) is known for its neuroprotective and neuroregenerative properties. EPO topical ocular administration has not been tested yet and its bioavailability could be improved by mucoadhesive hydrogels. Thus, this study aimed to develop and evaluate a chitosan (CS) and hyaluronic acid (HA) nanoparticulate system for topical ocular delivery of EPO. Nanoparticles were prepared by ionotropic gelation using six different HAs (HA1-HA6), and characterized by size, zeta potential (ZP), polydispersity index (Pdi), cytotoxicity and mucoadhesion. Encapsulation efficiency and drug loading capacity were also determined. Ex vivo permeation was tested using fresh porcine corneas, scleras and conjunctivas. The permeated EPO was quantified by ELISA, and its presence in the membranes was confirmed by immunohistochemistry. Nanoparticles (NPs) presented size ≤300 nm, ZP around +30 mV and low Pdi (0.167–0.539) at a 1:1 CS:HA mass ratio. The most suitable HA was HA6 (300 kDa – Eye), which had the best mucoadhesive properties. CS/HA6-EPO nanoformulation permeated more rapidly through porcine conjunctiva, followed by sclera and thirdly by cornea, as assessed by immunohistochemistry. All formulations were noncytotoxic on ARPE-19 and HaCaT cell lines, as evaluated by metabolic and membrane integrity tests. In conclusion, CS/HA6-EPO NPs could be a promising formulation for increasing EPO ocular bioavailability by enhancing its retention time and permeation through the different ocular membranes.pt_PT
dc.description.sponsorshipBeatriz Silva acknowledges the FCT fellowship SFRH/BD/130476/2017. The authors thank Inquiaroma (Barcelona, Spain) for the kind gift of all the brands of hyaluronic acid. This work was supported by the Fundação para a Ciência e a Tecnologia (FCT), Portugal (UID/DTP/04138/2013 to iMed.ULisboa, and UID/CVT/276/2013 to CIISA).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva B, Marto J, Braz BS, Delgado E, Almeida AJ, Gonçalves L. New nanoparticles for topical ocular delivery of erythropoietin. International Journal of Pharmaceutics [Internet]. fevereiro de 2020;576:119020. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S0378517320300028pt_PT
dc.identifier.doi10.1016/j.ijpharm.2020.119020pt_PT
dc.identifier.eid2-s2.0-85077974494
dc.identifier.issn0378-5173
dc.identifier.slugcv-prod-2118940
dc.identifier.urihttp://hdl.handle.net/10451/62013
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationUID/CVT/276/2013pt_PT
dc.relationRetinal erythropoietin distribution and neuroprotective effect in a nanoparticulate drug delivery system after subconjunctival and topical adminstration in an animal glaucoma model
dc.relationResearch Institute for Medicines
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0378517320300028pt_PT
dc.subjectErythropoietinpt_PT
dc.subjectChitosanpt_PT
dc.subjectHyaluronic acidpt_PT
dc.subjectNanoparticlespt_PT
dc.subjectOphthalmologypt_PT
dc.subjectEx vivo modelpt_PT
dc.titleNew nanoparticles for topical ocular delivery of erythropoietinpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberSFRH/BD/130476/2017
oaire.awardNumberUID/DTP/04138/2013
oaire.awardTitleRetinal erythropoietin distribution and neuroprotective effect in a nanoparticulate drug delivery system after subconjunctival and topical adminstration in an animal glaucoma model
oaire.awardTitleResearch Institute for Medicines
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F130476%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2013/PT
oaire.citation.startPage119020pt_PT
oaire.citation.titleInternational Journal of Pharmaceuticspt_PT
oaire.citation.volume576pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMarques Marto
person.familyNameLeitão das Neves Almeida
person.familyNameDiogo Gonçalves
person.givenNameJoana
person.givenNameAntónio José
person.givenNameLídia Maria
person.identifierI-6590-2012
person.identifier.ciencia-id891B-12E1-C2BF
person.identifier.ciencia-id8312-F853-C47A
person.identifier.ciencia-id7211-22BA-86AD
person.identifier.orcid0000-0001-5523-5622
person.identifier.orcid0000-0002-7807-4726
person.identifier.orcid0000-0002-6799-2740
person.identifier.scopus-author-id57195052432
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid7211-22BA-86AD | Lídia Maria Diogo Gonçalves
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication07221f19-3d9a-4bd5-88c4-1e066b49856f
relation.isAuthorOfPublicationdab457ee-7a1d-4c3d-a906-1e8df92fffd6
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