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Molecular and functional evidence for activity of murine IL-7 on human lymphocytes

2006Journal article Restricted access
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dc.contributor.authorBarata, João T.
dc.contributor.authorSilva, Ana
dc.contributor.authorAbecasis, Miguel
dc.contributor.authorCarlesso, Nadia
dc.contributor.authorCumano, Ana
dc.contributor.authorCardoso, Angelo A.
dc.date.accessioned2021-05-13T12:55:36Z
dc.date.available2021-05-13T12:55:36Z
dc.date.issued2006
dc.description© 2006 International Society for Experimental Hematology. Published by Elsevier Inc.pt_PT
dc.description.abstractAlthough interleukin-7 (IL-7) is essential for human and murine lymphopoiesis and homeostasis, clear disparities between these species regarding the role of IL-7 during B-cell development suggest that other, subtler differences may exist. One basic unsolved issue of IL-7 biology concerns cross-species activity, because in contrast to the human ortholog, the ability of murine (m)IL-7 to stimulate human cells remains unresolved. Establishing whether two-way cross-species reactivity occurs is fundamental for evaluating the role of IL-7 in chimeric human-mouse models, which are the most versatile tools for studying human lymphoid development and disease in vivo. Here, we show that mIL-7 triggers the same signaling pathways as human (h)IL-7 in human T cells, promoting similar changes in viability, proliferation, size, and immunophenotype, even at low concentrations. This ability is not confined to T cells, because mIL-7 mediates cell growth and protects human B-cell precursors from dexamethasone-induced apoptosis. Importantly, endogenous mIL-7 produced in the mouse thymic microenvironment stimulates human T cells, because their expansion in chimeric fetal thymic organ cultures is inhibited by a mIL-7-specific neutralizing antibody. Our results demonstrate that mIL-7 affects human lymphocytes and indicate that mouse models of human lymphoid development and disease must integrate the biological effects of endogenous IL-7 on human cells.pt_PT
dc.description.sponsorshipThis work was supported by grants POCTI/CBO/34914 and POCI/SAU-OBS/58913 from Fundação para a Ciência e a Tecnologia (FCT), Portugal. JTB and AS were supported by FCT fellowships SFRH/BPD/9436/2002 and SFRH/BD/18388/2004, respectively.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationExp Hematol. 2006 Sep;34(9):1133-1142pt_PT
dc.identifier.doi10.1016/j.exphem.2006.05.001pt_PT
dc.identifier.eissn1873-2399
dc.identifier.issn0301-472X
dc.identifier.urihttp://hdl.handle.net/10451/47833
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationIL-7- AND PI3K-MEDIATED SIGNALING IN T-CELL LEUKEMIA: THE ROLE OF EXTERNAL AND CELL-AUTONOMOUS SIGNALS IN TUMORIGENESIS
dc.relationTARGETING SIGNALING PATHWAYS AS THERAPY FOR HUMAN CANCER
dc.relation.publisherversionhttps://www.exphem.org/pt_PT
dc.titleMolecular and functional evidence for activity of murine IL-7 on human lymphocytespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleIL-7- AND PI3K-MEDIATED SIGNALING IN T-CELL LEUKEMIA: THE ROLE OF EXTERNAL AND CELL-AUTONOMOUS SIGNALS IN TUMORIGENESIS
oaire.awardTitleTARGETING SIGNALING PATHWAYS AS THERAPY FOR HUMAN CANCER
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F9436%2F2002/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F18388%2F2004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FCBO%2F34914%2F2000/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FSAU-OBS%2F58913%2F2004/PT
oaire.citation.endPage1141pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage1132pt_PT
oaire.citation.titleExperimental Hematologypt_PT
oaire.citation.volume34pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamPOCI
oaire.fundingStreamPOCI
person.familyNameBarata
person.givenNameJoão
person.identifier.orcid0000-0002-4826-8976
person.identifier.ridD-9181-2015
person.identifier.scopus-author-id7006937224
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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