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Guar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolates

2016Journal article Open access
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dc.contributor.authorSantos, Raquel
dc.contributor.authorGomes, Diana
dc.contributor.authorMacedo, Hermes
dc.contributor.authorBarros, Diogo
dc.contributor.authorTibério, Catarina
dc.contributor.authorVeiga, Ana Salomé
dc.contributor.authorTavares, Luís
dc.contributor.authorCastanho, Miguel A. R. B.
dc.contributor.authorOliveira, Manuela
dc.date.accessioned2022-02-22T16:13:12Z
dc.date.available2022-02-22T16:13:12Z
dc.date.issued2016
dc.description© 2016 The Authorspt_PT
dc.description.abstractDiabetic patients frequently develop diabetic foot ulcers (DFUs), particularly those patients vulnerable to Staphylococcus aureus opportunistic infections. It is urgent to find new treatments for bacterial infections. The antimicrobial peptide (AMP) nisin is a potential candidate, mainly due to its broad spectrum of action against pathogens. Considering that AMP can be degraded or inactivated before reaching its target at therapeutic concentrations, it is mandatory to establish effective AMP delivery systems, with the natural polysaccharide guar gum being one of the most promising. We analysed the antimicrobial potential of nisin against 23 S. aureus DFU biofilm-producing isolates. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were determined for nisin diluted in HCl and incorporated in guar gum gel. Statistical analysis was performed using the Wilcoxon matched-pair test. Nisin was effective against all isolates, including some multidrug-resistant clinical isolates, independent of whether it is incorporated in guar gum. While differences among MIC, MBC and MBIC values were observed for HCl- and guar gum- nisin, no significant differences were found between MBEC values. Inhibitory activity of both systems seems to differ only twofold, which does not compromise guar gum gel efficiency as a delivery system. Our results highlight the potential of nisin as a substitute for or complementary therapy to current antibiotics used for treating DFU infections, which is extremely relevant considering the increase in multidrug-resistant bacteria dissemination. The guar gum gel represents an alternative, practical and safe delivery system for AMPs, allowing the development of novel topical therapies as treatments for bacterial skin infections.pt_PT
dc.description.sponsorshipThe authors would like to acknowledge the Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa [Project UID/CVT/00276/2013, funded by Fundação para a Ciência e Tecnologia (FCT), Portugal]. This study was also conducted with the financial support of the project PTDC/SAU-MIC/122816/2010: ‘Biofilms in diabetic foot: microbial virulence characterization and cross-talk of major isolates’, funded by the FCT, Portugal. Raquel Santos, Diogo Barros and Ana Salomé Veiga acknowledge the FCT, Portugal, respectively, for two PhD fellowships (SFRH/BD/100571/2014 and PD/BD/113457/2015) and fellowship IF/00803/2012 under the FCT Investigator Programme.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJournal of Medical Microbiology (2016), 65, 1092–1099pt_PT
dc.identifier.doi10.1099/jmm.0.000329pt_PT
dc.identifier.eissn1473-5644
dc.identifier.issn0022-2615
dc.identifier.urihttp://hdl.handle.net/10451/51455
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMicrobiology Societypt_PT
dc.relationIF/00803/2012pt_PT
dc.relationUnraveling new therapies for diabetic foot ulcers: antimicrobial peptides and probiotics
dc.relationO impacto da urbanização na ecologia e comportamento de pequenos mamíferos
dc.relation.publisherversionhttps://www.microbiologyresearch.org/content/journal/jmmpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntimicrobial peptidept_PT
dc.subjectDiabetic foot ulcerpt_PT
dc.subjectGuar gumpt_PT
dc.subjectNisinpt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.titleGuar gum as a new antimicrobial peptide delivery system against diabetic foot ulcers Staphylococcus aureus isolatespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleUnraveling new therapies for diabetic foot ulcers: antimicrobial peptides and probiotics
oaire.awardTitleO impacto da urbanização na ecologia e comportamento de pequenos mamíferos
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FCVT%2F00276%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-MIC%2F122816%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F100571%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/PD%2FBD%2F113457%2F2015/PT
oaire.citation.endPage1099pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage1092pt_PT
oaire.citation.titleJournal of Medical Microbiologypt_PT
oaire.citation.volume65pt_PT
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
oaire.fundingStreamFARH
person.familyNameMartins dos Santos
person.familyNameVeiga
person.familyNameCastanho
person.givenNameTânia Raquel
person.givenNameAna Salome
person.givenNameMiguel
person.identifier.ciencia-id4F15-BEED-0A97
person.identifier.orcid0000-0003-2215-0467
person.identifier.orcid0000-0002-9892-2243
person.identifier.orcid0000-0001-7891-7562
person.identifier.scopus-author-id56745037100
person.identifier.scopus-author-id56605575600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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