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Grapevine aquaporins: gating of a tonoplast intrinsic protein (TIP2; 1) by cytosolic pH

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dc.contributor.authorLeitão, Luís
dc.contributor.authorPrista, Catarina
dc.contributor.authorMoura, Teresa F.
dc.contributor.authorLoureiro-Dias, Maria
dc.contributor.authorSoveral, Graça
dc.date.accessioned2019-03-21T10:53:56Z
dc.date.available2019-03-21T10:53:56Z
dc.date.issued2012
dc.description.abstractGrapevine (Vitis vinifera L.) is one of the oldest and most important perennial crops being considered as a fruit ligneous tree model system in which the water status appears crucial for high fruit and wine quality, controlling productivity and alcohol level. V. vinifera genome contains 28 genes coding for aquaporins, which acting in a concerted and regulated manner appear relevant for plant withstanding extremely unfavorable drought conditions essential for the quality of berries and wine. Several Vv aquaporins have been reported to be expressed in roots, shoots, berries and leaves with clear cultivar differences in their expression level, making their in vivo biochemical characterization a difficult task. In this work V. vinifera cv. Touriga nacional VvTnPIP1;1, VvTnPIP2;2 and VvTnTIP2;1 were expressed in yeast and water transport activity was characterized in intact cells of the transformants. The three aquaporins were localized in the yeast plasma membrane but only VvTnTIP2;1 expression enhanced the water permeability with a concomitant decrease of the activation energy of water transport. Acidification of yeast cytosol resulted in loss of VvTnTIP2;1 activity. Sequence analysis revealed the presence of a His131 residue, unusual in TIPs. By site directed mutagenesis, replacement of this residue by aspartic acid or alanine resulted in loss of pHin dependence while replacement by lysine resulted in total loss of activity. In addition to characterization of VvTn aquaporins, these results shed light on the gating of a specific tonoplast aquaporin by cytosolic pHpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLeita˜o L, Prista C, Moura TF, Loureiro-Dias MC, Soveral G (2012) Grapevine Aquaporins: Gating of a Tonoplast Intrinsic Protein (TIP2;1) by Cytosolic pH. PLoS ONE 7(3): e33219pt_PT
dc.identifier.doidoi:10.1371/journal.pone.0033219pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/17651
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPlos ONEpt_PT
dc.relationPOCTI/AGR/57403/2004pt_PT
dc.subjectgrapevinept_PT
dc.subjectaquaporinspt_PT
dc.titleGrapevine aquaporins: gating of a tonoplast intrinsic protein (TIP2; 1) by cytosolic pHpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPTDC/AGR-AAM/099154/2008
oaire.awardNumberSFRH/BPD/32511/2006
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-AAM%2F099154%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F32511%2F2006/PT
oaire.citation.titlePLoS ONEpt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNamePrista
person.familyNameLoureiro-Dias
person.givenNameCatarina
person.givenNameMaria
person.identifier23269
person.identifier.ciencia-id3C19-C25A-182D
person.identifier.orcid0000-0001-9307-1905
person.identifier.orcid0000-0002-6340-2416
person.identifier.ridN-7079-2013
person.identifier.ridN-7331-2013
person.identifier.scopus-author-id6603433211
person.identifier.scopus-author-id7003888372
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication344c4593-4c36-41ca-ab28-8ea76f20c5b5
relation.isAuthorOfPublication718c0dc9-c89f-444d-97fc-b8aa6f1edb57
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