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Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets

2021-01-19Journal article Open access
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dc.contributor.authorda Silva, Inês V.
dc.contributor.authorSoares, Bárbara P.
dc.contributor.authorPimpão, Catarina
dc.contributor.authorPinto, Rui M. A.
dc.contributor.authorCosta, Teresa
dc.contributor.authorFreire, João P. B.
dc.contributor.authorCorrent, Etienne
dc.contributor.authorChalvon-Demersay, Tristan
dc.contributor.authorPrates, José A.M
dc.contributor.authorLopes, Paula A.
dc.contributor.authorSoveral, Graça
dc.date.accessioned2022-01-30T22:52:12Z
dc.date.available2022-01-30T22:52:12Z
dc.date.issued2021-01-19
dc.descriptionResearch Areas: Science & Technology ; Other Topicspt_PT
dc.description.abstractABSTRACT - The regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that the amino acid glutamine is a physiological regulator of gluconeogenesis, whereas cystine promotes adiposity, herein we investigated the effects of dietary supplementation with glutamine and cystine on the serum biochemical parameters of piglets fed on amino acid-enriched diets, as well as on the transcriptional profile of membrane water and glycerol channels aquaporins (AQPs) in the ileum portion of the small intestine and its impact on intestinal permeability. Twenty male piglets with an initial body weight of 8.8 ± 0.89 kg were allocated to four dietary treatments (n = 5) and received, during a four week-period, a basal diet without supplementation (control) or supplemented with 8 kg/ton of glutamine (Gln), cystine (Cys) or the combination of the two amino acids in equal proportions (Gln + Cys). Most biochemical parameters were found improved in piglets fed Gln and Cys diet. mRNA levels of AQP3 were found predominant over the others. Both amino acids, individually or combined, were responsible for a consistent downregulation of AQP1, AQP7 and AQP10, without impacting on water permeability. Conversely, Cys enriched diet upregulated AQP3 enhancing basolateral membranes glycerol permeability and downregulating glycerol kinase (GK) of intestinal cells. Altogether, our data reveal that amino acids dietary supplementation can modulate intestinal AQPs expression and unveil AQP3 as a promising target for adipogenesis regulation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDa Silva IV, Soares BP, Pimpão C, Pinto RMA, Costa T, Freire JPB, Corrent E, Chalvon-Demersay T, Prates JAM, et al. 2021. Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets. PLoS ONE 16(1):e0245739. Doi: 10.1371/journal.pone.0245739pt_PT
dc.identifier.doi10.1371/journal.pone.0245739pt_PT
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.5/23318
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPublic Library Sciencept_PT
dc.relationDL57/2016/CP1438/CT0007pt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationResearch Institute for Medicines
dc.relationResearch Institute for Medicines
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245739pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBasolateral membrane-vesiclespt_PT
dc.subjectBrush-borderpt_PT
dc.subjectWater channelpt_PT
dc.subjectPermeabilitypt_PT
dc.subjectGlutathionept_PT
dc.subjectPlasmapt_PT
dc.subjectPigspt_PT
dc.subjectSupplementationpt_PT
dc.subjectMetabolismpt_PT
dc.subjectDiseasept_PT
dc.titleGlutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of pigletspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleResearch Institute for Medicines
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTM-SAL%2F28977%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04138%2F2020/PT
oaire.citation.conferencePlaceSan Franciscopt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volumeVol. 16(1):0245739pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVieira da Silva
person.familyNameMestre Prates
person.givenNameInês Margarida
person.givenNameJosé António
person.identifier.ciencia-id8A1D-AA13-FA0B
person.identifier.ciencia-id2617-53FF-04CD
person.identifier.orcid0000-0002-2916-4848
person.identifier.orcid0000-0003-1032-5987
person.identifier.ridK-9934-2013
person.identifier.scopus-author-id57195563908
person.identifier.scopus-author-id56003169500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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