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Antiviral activity of brequinar against African swine fever virus infection in vitro

2022-05-23Artigo científico Acesso aberto
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dc.contributor.authorGrigoryan, Rafayela
dc.contributor.authorArabyan, Erik
dc.contributor.authorIzmailyan, Roza
dc.contributor.authorKaralyan, Zaven
dc.contributor.authorJordão, Nuno
dc.contributor.authorFerreira, Fernando
dc.contributor.authorZakaryan, Hovakim
dc.date.accessioned2022-08-22T18:22:49Z
dc.date.available2022-08-22T18:22:49Z
dc.date.issued2022-05-23
dc.descriptionResearch Areas: Virologypt_PT
dc.description.abstractAfrican swine fever virus (ASFV) is a double-stranded DNA virus that causes an acute and hemorrhagic disease in domestic swine, resulting in significant economic losses to the global porcine industry. The lack of vaccines and antiviral drugs highlights the urgent need for antiviral studies against ASFV. Here, we report that brequinar (BQR), which is a specific inhibitor of dihydroorotate dehydrogenase, robustly inhibits ASFV replication in Vero cells, as well as in porcine macrophages. We demonstrate that BQR exerts its antiviral activity in a dosedependent manner through the depletion of pyrimidine pool. Although BQR does not affect the synthesis of an early viral protein, pI215L, the synthesis of late viral proteins, p17 and p72, is suppressed in the presence of BQR. We also show that BQR is able to induce cellular antiviral response in ASFV-infected macrophages by enhancing the expression of interferon-stimulated genes. Taken together, our study reveals that targeting nucleotide biosynthesis represents a promising strategy for developing antiviral agents against ASFV.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGrigoryan R, Arabyan E, Izmailyan R, Karalyan Z, Jordao N, Ferreira F, Zakaryan H. 2022. Antiviral activity of brequinar against African swine fever virus infection in vitro. Virus Research, 317:198826. DOI: https://doi.org/10.1016/j.virusres.2022.198826pt_PT
dc.identifier.doihttps://doi.org/10.1016/j.virusres.2022.198826pt_PT
dc.identifier.eissn1872-7492
dc.identifier.issn0168-1702
dc.identifier.urihttp://hdl.handle.net/10400.5/25202
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation20TTSG-1F007 (Science Committee of the Ministry of Education, Science, Culture and Sport RA)pt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0168170222001538?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAfrican swine fever viruspt_PT
dc.subjectAntiviralpt_PT
dc.subjectBrequinarpt_PT
dc.subjectPyrimidinept_PT
dc.titleAntiviral activity of brequinar against African swine fever virus infection in vitropt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/00276/2020
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.citation.conferencePlaceAmsterdam, Netherlandspt_PT
oaire.citation.titleVirus Researchpt_PT
oaire.citation.volume317:198826pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNamede Oliveira Jordão
person.familyNameFerreira
person.givenNameNuno Ricardo
person.givenNameFernando António da Costa
person.identifier.ciencia-idA61C-6451-F1F5
person.identifier.ciencia-idF81F-D7D4-57C5
person.identifier.orcid0000-0002-3803-966X
person.identifier.orcid0000-0001-5765-576X
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication02abcda7-14c1-467a-9c20-32d96907ea3a
relation.isAuthorOfPublication84ebf7d6-d393-4bf7-85f5-58a909ce1ff0
relation.isAuthorOfPublication.latestForDiscovery02abcda7-14c1-467a-9c20-32d96907ea3a
relation.isProjectOfPublication8563a044-0c86-49a8-a275-8b1bb62cc0d8
relation.isProjectOfPublication.latestForDiscovery8563a044-0c86-49a8-a275-8b1bb62cc0d8

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