Publication
Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization
| dc.contributor.author | Pereira, João R. | |
| dc.contributor.author | Araújo, Diana | |
| dc.contributor.author | Freitas, Patrícia | |
| dc.contributor.author | Marques, Ana C. | |
| dc.contributor.author | Delgado Alves, Vitor | |
| dc.contributor.author | Sevrin, Chantal | |
| dc.contributor.author | Grandfils, Christian | |
| dc.contributor.author | Fortunato, Elvira | |
| dc.contributor.author | Reis, Maria A.M. | |
| dc.contributor.author | Freitas, Filomena | |
| dc.date.accessioned | 2022-03-15T09:50:37Z | |
| dc.date.available | 2023-01-01T01:30:24Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated on apple pulp, a glucose- and fructoserich waste generated during juice production, to produce medium-chain length polyhydroxyalkanoates. A cell dry mass of 8.74 ± 0.20 g/L, with a polymer content of 49.25 ± 4.08% were attained. The produced biopolymer was composed of 42.7±0.1mol% 3-hydroxydecanoate, 17.9±1.0mol% 3-hydroxyoctanoate, 14.5±1.1mol% 3- hydroxybutyrate, 11.1±0.6 mol% 3-hydroxytetradecanoate, 10.1±0.5 mol% 3-hydroxydodecanoate and 3.7± 0.2 mol% 3-hydroxyhexanoate. It presented low glass transition and melting temperatures (−40.9 ± 0.7 °C and 42.0±0.1 °C, respectively), and a degradation temperature of 300.0±0.1 °C, coupled to a lowcrystallinity index (12.7±2.7%), amolecularweight (Mw) of 1.34 × 105±0.18×105 Da and a polydispersity index of 2.70±0.03. The biopolymer's filmswere dense and had a smooth surface, as demonstrated by Scanning ElectronMicroscopy. They presented a tension at break of 5.21 ± 1.09 MPa, together with an elongation of 400.5 ± 55.8% and an associated Young modulus of 4.86 ± 1.49 MPa, under tensile tests. These attractive filming properties of this biopolymer could potentially be valorised in several areas such as the fine chemicals industry, biomedicine, pharmaceuticals, or food packaging | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Pereira JR, Araújo D, Freitas P, Marques AC, Alves VD, Sevrin C, Grandfils C, Fortunato E, Reis MAM, Freitas F (2021). Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization. International Journal of Biological Macromolecules, 167:85-92 | pt_PT |
| dc.identifier.doi | https://doi.org/10.1016/j.ijbiomac.2020.11.162 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.5/23782 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation | UID/04378/2020 | pt_PT |
| dc.relation | UIDB/ 50025/2020 | pt_PT |
| dc.relation | UID/AGR/04129/2020 | pt_PT |
| dc.subject | fruit pulp waste | pt_PT |
| dc.subject | polyhydroxyalkanoates (PHA) | pt_PT |
| dc.subject | Pseudomonas chlororaphis | pt_PT |
| dc.title | Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.title | International Journal of Biological Macromolecules | pt_PT |
| person.familyName | Delgado Alves | |
| person.givenName | Vitor | |
| person.identifier | 1916677 | |
| person.identifier.ciencia-id | 2E1E-6918-4DB3 | |
| person.identifier.orcid | 0000-0002-4117-5582 | |
| person.identifier.scopus-author-id | 24536716000 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 8d1a8489-8789-4569-89e2-0bade23dd945 | |
| relation.isAuthorOfPublication.latestForDiscovery | 8d1a8489-8789-4569-89e2-0bade23dd945 |