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Bacterial persistence is essential for susceptible cell survival in indirect resistance, mainly for lower cell densities

dc.contributor.authorRebelo, João
dc.contributor.authorDomingues, Célia P. F.
dc.contributor.authorMonteiro, Francisca
dc.contributor.authorNogueira, Teresa
dc.contributor.authorDionisio, Francisco
dc.date.accessioned2021-09-08T13:07:08Z
dc.date.available2021-09-08T13:07:08Z
dc.date.issued2021-09-02
dc.description.abstractAntibiotic-susceptible bacteria may survive bactericidal antibiotics if other co-inhabiting bacteria detoxify the medium through antibiotic degradation or modification, a phenomenon denominated as indirect resistance. However, it is unclear how susceptible cells survive while the medium is still toxic. One explanation relies on the speed of detoxification, and another, non-exclusive explanation, relies on persistence, a state of bacterial dormancy where cells with low metabolic activity and growth rates are phenotypically tolerant to antibiotics and other cytotoxic substances. Here we simulated the fate of susceptible cells in laboratory experiments in the context of indirect resistance to understand whether persistence is necessary to explain the survival of susceptible cells. Depending on the strain and experimental conditions, the decay of persister populations may follow an exponential or a power-law distribution. Therefore, we studied the impact of both distributions in the simulations. Moreover, we studied the impact of considering that persister cells have a mechanism to sense the presence of a toxic substance–a mechanism that would enable cells to leave the dormant state when the medium becomes nontoxic. The simulations show that surviving susceptible cells under indirect resistance may originate both from persister and non-persister populations if the density of detoxifying cells is high. However, persistence was necessary when the initial density of detoxifying cells was low, although persister cells remained in that dormancy state for just a few hours. Finally, the results of our simulations are consistent both with exponential and power-law decay of the persistence population. Whether indirect resistance involves persistence should impact antibiotic treatments.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1371/journal.pone.0246500pt_PT
dc.identifier.urihttp://hdl.handle.net/10451/49455
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relationFCT ALG-01- 0145-FEDER-028824pt_PT
dc.relationFCT PTDC/BIA-MIC/28824/ 2017pt_PT
dc.relationFCT SFRH/BPD/ 123504/2016pt_PT
dc.relationFCT UIDB/00329/2020pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectantibioticspt_PT
dc.subjectantibiotic resistancept_PT
dc.subjectdetoxificationpt_PT
dc.subjectcomputer applicationspt_PT
dc.subjectgeneticspt_PT
dc.subjectradiipt_PT
dc.subjectEscherichia colipt_PT
dc.subjectplasmidspt_PT
dc.titleBacterial persistence is essential for susceptible cell survival in indirect resistance, mainly for lower cell densitiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue16pt_PT
oaire.citation.startPagee0246500pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume9pt_PT
person.familyNameRebelo
person.familyNameDomingues
person.familyNameSarreira Simões Horta Monteiro
person.familyNameVitorino Nogueira
person.familyNameDionísio
person.givenNameJoão
person.givenNameCélia
person.givenNameFrancisca
person.givenNameTeresa Isabel
person.givenNameFrancisco
person.identifierG-2273-2010
person.identifierA-2129-2009
person.identifier.ciencia-id3416-6299-1379
person.identifier.ciencia-idA815-CE3A-53C9
person.identifier.ciencia-idF211-4E18-2C69
person.identifier.ciencia-id7413-DB46-16E0
person.identifier.orcid0000-0002-6860-0446
person.identifier.orcid0000-0003-2970-010X
person.identifier.orcid0000-0002-5275-714X
person.identifier.orcid0000-0002-0059-5177
person.identifier.orcid0000-0002-3653-1511
person.identifier.scopus-author-id7004351448
person.identifier.scopus-author-id6506790127
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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