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Tumor-infiltrating T cells in skin basal cell carcinomas and squamous cell carcinomas: global Th1 preponderance with Th17 enrichment: a cross-sectional study

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dc.contributor.authorCunha, Daniela
dc.contributor.authorNeves, Marco
dc.contributor.authorSilva, Daniela
dc.contributor.authorSilvestre, Ana Rita
dc.contributor.authorBorralho, Paula
dc.contributor.authorArrobas, Fernando
dc.contributor.authorRibot, Julie
dc.contributor.authorFerreira, Fernando
dc.contributor.authorMoita, Luís F.
dc.contributor.authorSoares-de-Almeida, Luís
dc.contributor.authorMaia Silva, João Nuno
dc.contributor.authorFilipe, Paulo
dc.contributor.authorFerreira, João
dc.date.accessioned2024-07-24T11:40:32Z
dc.date.available2024-07-24T11:40:32Z
dc.date.issued2024
dc.description© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractBasal cell carcinomas (BCCs) and squamous cell carcinomas (SCCs) are high-incidence, non-melanoma skin cancers (NMSCs). The success of immune-targeted therapies in advanced NMSCs led us to anticipate that NMSCs harbored significant populations of tumor-infiltrating lymphocytes with potential anti-tumor activity. The main aim of this study was to characterize T cells infiltrating NMSCs. Flow cytometry and immunohistochemistry were used to assess, respectively, the proportions and densities of T cell subpopulations in BCCs (n = 118), SCCs (n = 33), and normal skin (NS, n = 30). CD8+ T cells, CD4+ T cell subsets, namely, Th1, Th2, Th17, Th9, and regulatory T cells (Tregs), CD8+ and CD4+ memory T cells, and γδ T cells were compared between NMSCs and NS samples. Remarkably, both BCCs and SCCs featured a significantly higher Th1/Th2 ratio (~four-fold) and an enrichment for Th17 cells. NMSCs also showed a significant enrichment for IFN-γ-producing CD8+T cells, and a depletion of γδ T cells. Using immunohistochemistry, NMSCs featured denser T cell infiltrates (CD4+, CD8+, and Tregs) than NS. Overall, these data favor a Th1-predominant response in BCCs and SCCs, providing support for immune-based treatments in NMSCs. Th17-mediated inflammation may play a role in the progression of NMSCs and thus become a potential therapeutic target in NMSCs.pt_PT
dc.description.sponsorshipThis work was funded by Almirall, S.A. (FPJ001079; IMM/BI/82-2017); Laboratoires Dermatologiques D’Uriage Portugal, S.A.; Novartis Farma—Produtos Farmacêuticos, S.A.; Sanofi Genzyme; AbbVie Portugal; Naos Portugal; IFC Skincare Portugal, Lda; L’Oreal Portugal; FCT-Portugal (PTDC/MEC-DER/30198/2017 FARM ID). Dr. Neves and Dr. Silva received salary support from Almirall S.A. funding.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCells. 2024 Jun 3;13(11):964pt_PT
dc.identifier.doi10.3390/cells13110964pt_PT
dc.identifier.eissn2073-4409
dc.identifier.urihttp://hdl.handle.net/10451/65439
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPTDC/MEC-DER/30198/2017pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/journal/cellspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectTh1 cellspt_PT
dc.subjectTh17 cellspt_PT
dc.subjectTh2 cellspt_PT
dc.subjectBasal cell carcinomapt_PT
dc.subjectSquamous cell carcinomapt_PT
dc.subjectTumor-infiltrating lymphocytespt_PT
dc.titleTumor-infiltrating T cells in skin basal cell carcinomas and squamous cell carcinomas: global Th1 preponderance with Th17 enrichment: a cross-sectional studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue11pt_PT
oaire.citation.titleCellspt_PT
oaire.citation.volume13pt_PT
person.familyNameCasimiro Cunha
person.familyNameNeves
person.familyNameBorralho Nunes
person.familyNameRibot
person.familyNameFerreira
person.familyNameSoares-de-Almeida
person.familyNameMaia Silva
person.familyNameFilipe
person.familyNameFerreira
person.givenNameAna Daniela
person.givenNameMarco
person.givenNamePaula
person.givenNameJulie Cécile Caroline
person.givenNameFernando António da Costa
person.givenNameLuis
person.givenNameJoão Nuno
person.givenNamePaulo
person.givenNameJoão António Augusto
person.identifier.ciencia-idD61D-87EB-F9A3
person.identifier.ciencia-idE91A-B8A4-D3B9
person.identifier.ciencia-id7C1C-F771-97C9
person.identifier.ciencia-idF81F-D7D4-57C5
person.identifier.ciencia-id691E-9432-01C0
person.identifier.ciencia-id171A-309C-C397
person.identifier.ciencia-idEA1C-91BA-1C8B
person.identifier.orcid0000-0002-5472-0543
person.identifier.orcid0000-0002-5953-4416
person.identifier.orcid0000-0002-8556-2090
person.identifier.orcid0000-0002-7852-343X
person.identifier.orcid0000-0001-5765-576X
person.identifier.orcid0000-0003-4026-6105
person.identifier.orcid0000-0002-2326-3765
person.identifier.orcid0000-0002-7337-6493
person.identifier.ridJ-8716-2012
person.identifier.scopus-author-id57214563175
person.identifier.scopus-author-id6507304614
person.identifier.scopus-author-id7003448874
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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