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What Is the Impact of Antibiotic Resistance Determinants on the Bacterial Death Rate?

2025-02Journal article Open access
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dc.contributor.authorLuz, Bruno T. S.
dc.contributor.authorRebelo, João S.
dc.contributor.authorMonteiro, Francisca
dc.contributor.authorDionisio, Francisco
dc.date.accessioned2025-04-14T12:43:35Z
dc.date.available2025-04-14T12:43:35Z
dc.date.issued2025-02
dc.description.abstractObjectives: Antibiotic-resistant bacteria are widespread, with resistance arising from chromosomal mutations and resistance genes located in the chromosome or in mobile genetic elements. While resistance determinants often reduce bacterial growth rates, their influence on bacterial death under bactericidal antibiotics remains poorly understood. When bacteria are exposed to bactericidal antibiotics to which they are susceptible, they typically undergo a two-phase decline: a fast initial exponentially decaying phase, followed by a persistent slow-decaying phase. This study examined how resistance determinants affect death rates during both phases. Methods: We analyzed the death rates of ampicillin-exposed Escherichia coli populations of strains sensitive to ampicillin but resistant to nalidixic acid, rifampicin, or both, and bacteria carrying the conjugative plasmids RN3 or R702. Results: Single mutants resistant to nalidixic acid or rifampicin decayed faster than sensitive cells during the early phase, whereas the double-resistant mutant exhibited prolonged survival. These contrasting impacts suggest epistatic interactions between both chromosomal mutations. Persistent-phase death rates for chromosomal mutants did not differ significantly from wild-type cells. In contrast, plasmid-carrying bacteria displayed distinct dynamics: R702 plasmid-bearing cells showed higher persistent-phase death rates than plasmid-free cells, while RN3 plasmid-bearing cells exhibited lower rates. Conclusions: Bactericidal antibiotics may kill bacteria resistant to other antibiotics more effectively than wild-type cells. Moreover, epistasis may occur when different resistance determinants occur in the same cell, impacting the bactericidal potential of the antibiotic of choice. These results have significant implications for optimizing bacterial eradication protocols in clinical settings, as well as in animal health and industrial food safety management.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLuz, B.T.S.; Rebelo, J.S.; Monteiro, F.; Dionisio, F. What Is the Impact of Antibiotic Resistance Determinants on the Bacterial Death Rate? Antibiotics 2025, 14, 201. https://doi.org/10.3390/antibiotics14020201pt_PT
dc.identifier.doi10.3390/antibiotics14020201pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/100194
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationFCT-Fundação para a Ciência e a Tecnologia, IP for their fellowships (PhD grant SFRH/BD/04631/2021 and post doctoral grant SFRH/BPD/123504/2016).pt_PT
dc.relationFCT cE3c (DOI:10.54499/UIDB/00329/2020 and UID/00329/2025) and the associate lab CHANGE (LA/P/0121/2020).pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectbacterial deathpt_PT
dc.subjectbacterial persistencept_PT
dc.subjectantibiotic resistancept_PT
dc.subjectepistasispt_PT
dc.subjectbacterial fitnesspt_PT
dc.subjectconjugative plasmidpt_PT
dc.subjectnalidixic acidpt_PT
dc.subjectrifampicinpt_PT
dc.titleWhat Is the Impact of Antibiotic Resistance Determinants on the Bacterial Death Rate?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue2pt_PT
oaire.citation.startPage201pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume14pt_PT
person.familyNameRebelo
person.familyNameSarreira Simões Horta Monteiro
person.familyNameDionísio
person.givenNameJoão
person.givenNameFrancisca
person.givenNameFrancisco
person.identifierA-2129-2009
person.identifier.ciencia-idA015-FF75-397C
person.identifier.ciencia-idA815-CE3A-53C9
person.identifier.ciencia-id7413-DB46-16E0
person.identifier.orcid0000-0002-8662-7378
person.identifier.orcid0000-0002-5275-714X
person.identifier.orcid0000-0002-3653-1511
person.identifier.scopus-author-id6506790127
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication09097aa0-9d09-459f-a089-84f103805fa4
relation.isAuthorOfPublication8063a7ff-bf07-4b7d-83a4-61786b528709
relation.isAuthorOfPublicationaa2a19c2-e8bf-4b68-98f3-737d4515e13e
relation.isAuthorOfPublication.latestForDiscovery8063a7ff-bf07-4b7d-83a4-61786b528709

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