Logo do repositório
 
Publicação

Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability

2019-12-01Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorCota, João Bettencourt
dc.contributor.authorSilva, Vanessa Ferreira da
dc.contributor.authorChambel, Lélia
dc.contributor.authorVeloso, Maria Gabriela
dc.contributor.authorVieira-Pinto, Madalena
dc.contributor.authorOliveira, Manuela
dc.date.accessioned2021-06-03T23:54:27Z
dc.date.available2021-06-03T23:54:27Z
dc.date.issued2019-12-01
dc.descriptionMicrobiology / Veterinary Sciencespt_PT
dc.description.abstractABSTRACT - Pork is one of the most common vehicles of non-typhoid foodborne Salmonella, with the slaughterhouse representing a key point for the infection of pigs and carcass contamination. By comparing matching samples taken from animals at the dirty (skin) and clean (inner and outer carcass surface) areas of the slaughterline, this study aimed to assess potential Salmonella contamination routes of pig carcasses within a Portuguese abattoir. Forty-four Salmonella isolates were retrieved from 120 pigs, and further characterized through pheno and genotypical methods. Most frequent serotypes found were Salmonella 4, [5],12:i:- (47.7%), Salmonella Rissen (40.9%) and Salmonella Derby (11.4%). Isolates were most commonly collected from the skin of pigs sampled at the dirty area (59.1%), followed by the inner (38.1%) and outer (9.1%) carcass surface sampled at the clean area. Most isolates (79.5%) were considered to be multidrug resistant and all harbored the virulence associated genes invA, invH, sopB, stn, slyA, phoP, phoQ and agfA. PFGE analysis revealed that most bacterial isolates belonging to the same serotype, recovered from animals from different farms, and slaughtered at separate days were genetically undistinguishable. Furthermore, our findings suggest that Salmonella Rissen might have an increased ability to endure on the slaughterhouse environment when compared with the other serotypes. Concluding, this study shows that the slaughterhouse may be a key point for the dissemination of resistant and virulent Salmonella strains, which stresses the importance of the implementation of good hygiene practices at the slaughterhouse and of the application of corrective measures to avoid cross-contamination.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCota, João Bettencourt, Silva, Vanessa Ferreira da, Chambel, Lélia, Veloso, Maria Gabriela, Vieira-Pinto, Madalena, Oliveira, Manuela 2019. Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability [article]. Veterinary Microbiology, 239:108457. Doi:10.1016/j.vetmic.2019.108457pt_PT
dc.identifier.doi10.1016/j.vetmic.2019.108457pt_PT
dc.identifier.issn18732542
dc.identifier.urihttp://hdl.handle.net/10400.5/21427
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier B.V.pt_PT
dc.relationCenter for Interdisciplinary Research in Animal Health
dc.relationAnimal and Veterinary Research Centre
dc.relation.ispartofseries108457;
dc.relation.publisherversionhttps://reader.elsevier.com/reader/sd/pii/S0378113519311721?token=0C8302A066AC1BE996EAFB2D7E24304D1CA1DE19CAE6311DC874EA8414FB606141A532B5EB0C82BE81624D8E9324A187&originRegion=eu-west-1&originCreation=20210603235223pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCarcasspt_PT
dc.subjectContaminationpt_PT
dc.subjectPFGEpt_PT
dc.subjectPorkpt_PT
dc.subjectSalmonellapt_PT
dc.subjectSlaughterhousept_PT
dc.titlePheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence abilitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCenter for Interdisciplinary Research in Animal Health
oaire.awardTitleAnimal and Veterinary Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FCVT%2F00276%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FCVT%2F00772%2F2019/PT
oaire.citation.titleVeterinary Microbiologypt_PT
oaire.citation.volume239pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication56c41353-36ab-4809-855e-b7986e3e4bb1
relation.isProjectOfPublicationedd4fa87-6a0b-4869-b190-3f3d5a65f0a6
relation.isProjectOfPublication.latestForDiscoveryedd4fa87-6a0b-4869-b190-3f3d5a65f0a6

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Pheno and genotyping of Salmonella from slaughtered pigs in a Portuguese abattoir reveal differential persistence ability.pdf
Tamanho:
754.78 KB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CIISA - Artigos em revistas internacionais