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Cerebrospinal fluid chitinases as biomarkers for Amyotrophic Lateral Sclerosis

2021Artigo científico Acesso aberto
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dc.contributor.authorCosta, Julia
dc.contributor.authorGromicho, Marta
dc.contributor.authorPronto Laborinho, Ana Catarina
dc.contributor.authorAlmeida, Conceição
dc.contributor.authorGomes, Ricardo A.
dc.contributor.authorGuerreiro, Ana C. L.
dc.contributor.authorOliva, Abel
dc.contributor.authorPinto, Susana
dc.contributor.authorCarvalho, Mamede
dc.date.accessioned2021-09-08T14:27:57Z
dc.date.available2021-09-08T14:27:57Z
dc.date.issued2021
dc.description© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractAmyotrophic lateral sclerosis (ALS) is a neurodegenerative neuromuscular disease that affects motor neurons controlling voluntary muscles. Survival is usually 2-5 years after onset, and death occurs due to respiratory failure. The identification of biomarkers would be very useful to help in disease diagnosis and for patient stratification based on, e.g., progression rate, with implications in therapeutic trials. Neurofilaments constitute already-promising markers for ALS and, recently, chitinases have emerged as novel marker targets for the disease. Here, we investigated cerebrospinal fluid (CSF) chitinases as potential markers for ALS. Chitotriosidase (CHIT1), chitinase-3-like protein 1 (CHI3L1), chitinase-3-like protein 2 (CHI3L2) and the benchmark marker phosphoneurofilament heavy chain (pNFH) were quantified by an enzyme-linked immunosorbent assay (ELISA) from the CSF of 34 ALS patients and 24 control patients with other neurological diseases. CSF was also analyzed by UHPLC-mass spectrometry. All three chitinases, as well as pNFH, were found to correlate with disease progression rate. Furthermore, CHIT1 was elevated in ALS patients with high diagnostic performance, as was pNFH. On the other hand, CHIT1 correlated with forced vital capacity (FVC). The three chitinases correlated with pNFH, indicating a relation between degeneration and neuroinflammation. In conclusion, our results supported the value of CHIT1 as a diagnostic and progression rate biomarker, and its potential as respiratory function marker. The results opened novel perspectives to explore chitinases as biomarkers and their functional relevance in ALS.pt_PT
dc.description.sponsorshipWe acknowledge iNOVA4Health – UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior, through national funds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDiagnostics (Basel). 2021 Jul 5;11(7):1210pt_PT
dc.identifier.doi10.3390/diagnostics11071210pt_PT
dc.identifier.eissn2075-4418
dc.identifier.urihttp://hdl.handle.net/10451/49468
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/journal/diagnosticspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAmyotrophic lateral sclerosispt_PT
dc.subjectBiomarkerspt_PT
dc.subjectCerebrospinal fluidpt_PT
dc.subjectChitinasespt_PT
dc.titleCerebrospinal fluid chitinases as biomarkers for Amyotrophic Lateral Sclerosispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumber157561
oaire.awardNumber157874
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157561/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157874/PT
oaire.citation.issue7pt_PT
oaire.citation.titleDiagnosticspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCosta
person.familyNameSilva
person.familyNamePronto Laborinho
person.familyNamePinto
person.familyNamede Carvalho
person.givenNameJulia
person.givenNameMarta Luísa Gromicho Morgado
person.givenNameAna Catarina
person.givenNameSusana
person.givenNameMamede
person.identifierB-2171-2018
person.identifier593760
person.identifier.ciencia-idEA1D-1979-5C3A
person.identifier.ciencia-id891E-679B-B2DE
person.identifier.ciencia-idD31B-E89C-CB94
person.identifier.orcid0000-0001-7782-6319
person.identifier.orcid0000-0003-2111-4579
person.identifier.orcid0000-0002-2816-9698
person.identifier.orcid0000-0002-0727-5897
person.identifier.orcid0000-0001-7556-0158
person.identifier.ridB-7131-2008
person.identifier.ridL-8394-2019
person.identifier.scopus-author-id7402461218
person.identifier.scopus-author-id6506583274
person.identifier.scopus-author-id56177475200
person.identifier.scopus-author-id23397978500
person.identifier.scopus-author-id7101893769
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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