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Cell surface damage and morphological changes in Oenococcus oeni after freeze-drying and incubation in synthetic wine

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dc.contributor.authorBravo-Ferrada, Bárbara Mercedes
dc.contributor.authorAbreu, Sónia Gonçalves
dc.contributor.authorSemorile, Liliana
dc.contributor.authorSantos, Nuno C.
dc.contributor.authorBrizuela, Natalia S.
dc.contributor.authorElizabeth Tymczyszyn, E.
dc.contributor.authorHollmann, Axel
dc.date.accessioned2019-04-04T15:20:55Z
dc.date.available2019-04-04T15:20:55Z
dc.date.issued2018
dc.description© 2018 Elsevier Inc. All rights reserved.pt_PT
dc.description.abstractThe aim of the present study was to evaluate the effects of freeze-drying in the presence of trehalose as a cryoprotectant, followed by incubation in synthetic wine, on surface damage, viability and l-malic acid consumption of the oenological strain Oenococcus oeni UNQOe 73.2. After freeze-drying, no significant differences were observed in the number of viable cells (for both acclimated and non-acclimated cultures) respect to the fresh culture. In contrast, loss of viability was observed after wine incubation for 24 h, being acclimated freeze-dried cells the best conditions for this. After the preservation process, small changes in cell morphology were observed by Atomic Force Microscopy (AFM). The Zeta potential and AFM showed that 24 h of wine incubation was enough to induce several cell surface modifications. Plate count data allowed us to establish that surface damage is an important factor for loss of viability, regardless of the acclimation treatment. Although the number of surviving O. oeni cells decreased dramatically after incubation in synthetic wine for 15 days, the consumption of l-malic acid was higher than 70%, with freeze-dried cells showing a better performance than fresh cultures. These results demonstrate that O. oeni freeze-dried cultures could be applied to direct wine inoculation, to conduct malolactic fermentation, maintaining its technological properties and reducing the time and costs of the winemaking process.pt_PT
dc.description.sponsorshipThis work was funded by grants from Universidad Nacional de Quilmes, Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT – MINCyT, Argentina, PICT PICT 2013-1481, 2014-1395 and PICT 2016-3435), Comisión Nacional de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-BA, Argentina), and Fundação para a Ciência e a Tecnologia – Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal). NB has fellowships from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). BMBF, EET and AH are members of the Carrera del Investigador Científico y Tecnológico (CONICET, Argentina). LS is a member of the Research Career of Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-BA, Argentina).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCryobiology 82 (2018) 15–21pt_PT
dc.identifier.doi10.1016/j.cryobiol.2018.04.014pt_PT
dc.identifier.issn0011-2240
dc.identifier.urihttp://hdl.handle.net/10451/37851
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/cryobiologypt_PT
dc.subjectL-malic acid consumptionpt_PT
dc.subjectOenococcus oenipt_PT
dc.subjectFreeze-dryingpt_PT
dc.subjectAtomic force microscopypt_PT
dc.subjectZeta potentialpt_PT
dc.titleCell surface damage and morphological changes in Oenococcus oeni after freeze-drying and incubation in synthetic winept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage21pt_PT
oaire.citation.startPage15pt_PT
oaire.citation.titleCryobiologypt_PT
oaire.citation.volume82pt_PT
person.familyNameAbreu
person.familyNameSantos
person.familyNameHollmann
person.givenNameSónia
person.givenNameNuno
person.givenNameAxel
person.identifier198825
person.identifier.ciencia-idF811-74AE-412A
person.identifier.ciencia-idCD13-5E3A-A3C5
person.identifier.ciencia-id9E16-D9F8-1807
person.identifier.orcid0000-0003-2903-4282
person.identifier.orcid0000-0002-0580-0475
person.identifier.orcid0000-0002-8060-2997
person.identifier.ridH-9482-2017
person.identifier.ridN-7248-2013
person.identifier.scopus-author-id35602210000
person.identifier.scopus-author-id55940818300
person.identifier.scopus-author-id15848341400
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc077a1e4-3d11-47c7-b5d4-29a5a4190a35
relation.isAuthorOfPublication4656d912-5a2d-4c80-8921-2bcba3aa441a
relation.isAuthorOfPublication01aa58e0-78b7-46d8-ad08-2cd9c71c1510
relation.isAuthorOfPublication.latestForDiscoveryc077a1e4-3d11-47c7-b5d4-29a5a4190a35

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