| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 4.97 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Alternative oxidase (AOX) is a key enzyme of the alternative respiration, known to be
involved in plant development and in response to various stresses. To verify the role of DcAOX1 and
DcAOX2a genes in carrot tap root growth and in response to cold stress, their expression was analyzed
in two experiments: during root growth for 13 weeks and in response to a cold challenge trial of
7 days, in both cases using different carrot cultivars. Carrot root growth is initially characterized by an
increase in length, followed by a strong increase in weight. DcAOX2a presented the highest expression
levels during the initial stages of root growth for all cultivars, but DcAOX1 showed no particular
trend in expression. Cold stress had a negative impact on root growth, and generally up-regulated
DcAOX2a with no consistent effect on DcAOX1. The identification of cis-acting regulatory elements
(CAREs) located at the promoters of both genes showed putative sequences involved in cold stress
responsiveness, as well as growth. However, DcAOX2a promoter presented more CAREs related to
hormonal pathways, including abscisic acid and gibberellins synthesis, than DcAOX1. These results
point to a dual role of DcAOX2a on carrot tap root secondary growth and cold stress response
Description
Keywords
Daucus carota alternative oxidase growth chilling stress cis-elements
Pedagogical Context
Citation
Campos, M.D.; Campos, C.; Nogales, A.; Cardoso, H. Carrot AOX2a Transcript Profile Responds to Growth and Chilling Exposure. Plants 2021, 10, 2369
Publisher
MDPI