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Using zebrafish embryo bioassays combined with high-resolution mass spectrometry screening to assess ecotoxicological water bodies quality status : a case study in Panama rivers

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dc.contributor.authorWilson, Estibali Wilkie
dc.contributor.authorCastro, Verónica
dc.contributor.authorChaves, Raquel S.
dc.contributor.authorEspinosa, Miguel
dc.contributor.authorRodil, Rosario
dc.contributor.authorQuintana, José Benito
dc.contributor.authorVieira, Maria Natividade
dc.contributor.authorSantos, Miguel M.
dc.date.accessioned2021-02-22T17:05:13Z
dc.date.available2021-02-22T17:05:13Z
dc.date.issued2021
dc.descriptionCopyright © 2021 Elsevier Ltd. All rights reserved.pt_PT
dc.description.abstractSeveral studies show that many water bodies in developing countries are increasingly affected by anthropogenic pressure, such as agricultural activities, domestic and industrial wastewater. However, data is scarce in several of such countries, including Panama. Thus, in this work, the ecotoxicological status of selected rivers in Panama with distinct input sources were evaluated using the zebrafish (Danio rerio) embryo bioassays combined with a liquid chromatography-high resolution mass spectrometry screening of contaminants of emerging concern (CECs), using a library of over 3200 chemicals. A total of 68 CECs, including pharmaceuticals and metabolites, pesticides and several industrial chemicals, could be tentatively identified. Additionally, the zebrafish embryo bioassays showed a significant increase (p < 0.05) in embryo mortality/abnormalities when incubated with water samples from two rivers, Matasnillo and Curundú (47.5% and 32%, respectively). Importantly, a positive correlation between ecotoxicological endpoints and some of the detected CECs was observed. The findings demonstrate that both rivers are under strong anthropogenic pressure, and therefore, management actions are urgently needed to decrease their level of contamination. Overall, this study further supports the use of the zebrafish embryo bioassay as a fast, high throughput approach for screening the toxicity of water samples, and highlights the advantages of combining ecotoxicological assays with high-resolution mass spectrometry to an expedite assessment of the ecotoxicological status of water bodies.pt_PT
dc.description.sponsorshipThis work was supported by the Portuguese Foundation for Science and Technology (FCT) (PTDC|CTA-AMB|31554|2017; UIDB/04423/2020; UIDP/04423/2020), the Spanish Agencia Estatal de Investigacion (ref. CTM2017-84763-C3-2-R), the Galician Council of Culture, Education and Universities (ref. ED431C2017/36 and Veronica Castro predoctoral contract, ref. ED481A-2017/156), co-funded by the European Regional Development Fund (ERDF/FEDER).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationChemosphere. 2021 Feb 2;272:129823pt_PT
dc.identifier.doi10.1016/j.chemosphere.2021.129823pt_PT
dc.identifier.eissn1879-1298
dc.identifier.issn0045-6535
dc.identifier.urihttp://hdl.handle.net/10451/46461
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationTDC-CTA-AMB-31554-2017pt_PT
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationD431C2017/36pt_PT
dc.relationD481A-2017/156pt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/chemospherept_PT
dc.subjectDanio reriopt_PT
dc.subjectEmerging contaminantspt_PT
dc.subjectQuadrupole-time-of flight mass spectrometry (QTOF)pt_PT
dc.subjectRisk assessmentpt_PT
dc.subjectScreeningpt_PT
dc.subjectToxicity testingpt_PT
dc.titleUsing zebrafish embryo bioassays combined with high-resolution mass spectrometry screening to assess ecotoxicological water bodies quality status : a case study in Panama riverspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04423%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04423%2F2020/PT
oaire.citation.startPage129823pt_PT
oaire.citation.titleChemospherept_PT
oaire.citation.volume272pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameChaves
person.givenNameRaquel
person.identifier.ciencia-id4A1A-79C0-2F0D
person.identifier.orcid0000-0002-7118-1465
person.identifier.scopus-author-id57209010126
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication5e83d1ec-300b-4499-85d4-43c22feb0020
relation.isAuthorOfPublication.latestForDiscovery5e83d1ec-300b-4499-85d4-43c22feb0020
relation.isProjectOfPublication2cd83bc3-f92d-4fe2-a310-991848684e35
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relation.isProjectOfPublication.latestForDiscovery3970332f-1e78-4f79-a894-c7bf2d565450

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