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VIP enhances synaptic transmission to hippocampal CA1 pyramidal cells through activation of both VPAC1 and VPAC2 receptors

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dc.contributor.authorCunha-Reis, Diana
dc.contributor.authorRibeiro, Joaquim A.
dc.contributor.authorSebastião, Ana M
dc.date.accessioned2022-12-13T12:33:58Z
dc.date.available2022-12-13T12:33:58Z
dc.date.issued2005
dc.description© 2005 Published by Elsevier B.V.pt_PT
dc.description.abstractWe previously described that vasoactive intestinal peptide (VIP) increases synaptic transmission to hippocampal CA1 pyramidal cells at concentrations known to activate VIP-selective receptors (VPAC1 and VPAC2) but not the PACAP-selective PAC1 receptor. We now investigated the involvement of VPAC1 and VPAC2 receptors in the effects elicited by VIP as well as the transduction pathways activated by VIP to cause enhancement of synaptic transmission. Blockade of either VPAC1 or VPAC2 receptors with PG 97-269 (100 nM) or PG 99-465 (100 nM) inhibited VIP-induced enhancement of synaptic transmission. Selective activation of VPAC1 receptors with [K15, R16, L27] VIP(1-7)/GRF(8-27) (10 nM) or of VPAC2 receptors with RO 25-1553 (10 nM) increased synaptic transmission to CA1 pyramidal cells, and this increase was larger when both agonists were applied together. Inhibition of either PKA with H-89 (1 microM) or PKC with GF109203X (1 microM) attenuated the effect of VIP (1 nM). GF109203X (1 microM) abolished the effect of the VPAC1 agonist [K15, R16, L27] VIP(1-7)/GRF(8-27) (10 nM) on hippocampal synaptic transmission but that effect was not changed by H-89 (1 microM). The effect of RO 25-1553 (100 nM) obtained in the presence of both the PAC1 and VPAC1 antagonists, M65 (30 nM) and PG 97-269 (100 nM), was strongly inhibited by H-89 (1 microM) but not GF109203X (1 microM). It is concluded that VIP enhances synaptic transmission to CA1 pyramidal cell dendrites through VPAC1 and VPAC2 receptor activation. VPAC1-mediated actions are dependent on PKC activity, and VPAC2-mediated actions are responsible for the PKA-dependent actions of VIP on CA1 hippocampal transmission.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBrain Res. 2005 Jul 5;1049(1):52-60pt_PT
dc.identifier.doi10.1016/j.brainres.2005.04.077pt_PT
dc.identifier.eissn1872-6240
dc.identifier.issn0006-8993
dc.identifier.urihttp://hdl.handle.net/10451/55378
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/brain-researchpt_PT
dc.subjectVPAC1pt_PT
dc.subjectVPAC2pt_PT
dc.subjectPKApt_PT
dc.subjectPKCpt_PT
dc.subjectHippocampal slicespt_PT
dc.subjectfEPSPspt_PT
dc.titleVIP enhances synaptic transmission to hippocampal CA1 pyramidal cells through activation of both VPAC1 and VPAC2 receptorspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage60pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage52pt_PT
oaire.citation.titleBrain Researchpt_PT
oaire.citation.volume1049pt_PT
person.familyNameJerónimo da Cunha Reis
person.familyNameRibeiro
person.familyNameSebastião
person.givenNameDiana Lina
person.givenNameJoaquim
person.givenNameAna M
person.identifierF-1689-2011
person.identifier.ciencia-id1F1E-73C0-FD44
person.identifier.ciencia-id081F-2518-907F
person.identifier.ciencia-idF112-55E8-E37E
person.identifier.orcid0000-0002-0900-9306
person.identifier.orcid0000-0002-9330-3507
person.identifier.orcid0000-0001-9030-6115
person.identifier.scopus-author-id8571270200
person.identifier.scopus-author-id35498669400
person.identifier.scopus-author-id7004409879
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbd9c582c-30c6-4cf5-b91a-143562ce0931
relation.isAuthorOfPublication86da944c-5e6a-4ec5-a56e-4ed82ece7a17
relation.isAuthorOfPublication304abd7f-071b-4447-a8a3-4aa5f0547141
relation.isAuthorOfPublication.latestForDiscovery304abd7f-071b-4447-a8a3-4aa5f0547141

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