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Estudo de polimorfismos associados ao tipo de pelagem e doenças na raça Garrana
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Orientador(es)
Resumo(s)
A raça Garrana é uma das raças autóctones portuguesas de equinos classificada como ameaçada pela União Europeia. Por esta razão, torna-se fundamental preservar estes Recursos Genéticos Animais (RGAn), quer do ponto de vista da biodiversidade, quer da sua valorização económica.
A identificação dos equinos geneticamente superiores para determinadas caraterísticas é fundamental para a avaliação genética de programas de melhoramento genético e de conservação. Neste contexto, o presente trabalho teve como objetivo analisar os genótipos de equinos da raça Garrana em alguns marcadores genéticos associados a patologias e a caraterísticas de pelagem.
Para analisar os genótipos dos 109 equinos de raça Garrana em estudo, a extração de ADN foi feita segundo o método de extração salina High Salt Method (Montegomery & Sise, 1990). A Genotyping-by-sequencing (GBS) foi realizada através do AgriSeq Custom Panel - Equine 500 panel – Parentage & TD da Thermo Fisher Scientific, que permite conhecer o genótipo de equinos em 500 SNP distribuídos em diferentes genes. Neste trabalho foram apenas apresentados os resultados de 37 dos 500 SNP, em que 18 estão ligados à definição das pelagens dos equinos e os restantes 19 estão associados à resistência/suscetibilidade a doenças e à conformação dos animais.
Os equinos não apresentaram polimorfismo entre si para cada SNP. Relativamente aos SNP associados à definição das pelagens, os equinos analisados não apresentaram nenhum alelo alternativo, pelo que não deverão expressar as caraterísticas fenotípicas correlacionadas. No que diz respeito aos SNP ligados à resistência/suscetibilidade a doenças e a conformação dos animais, nenhum dos equinos revelou ser portador de alelos alternativos, não podendo, desta forma, transmitir à sua descendência o alelo associado às doenças relatadas.
The Garrana breed is one of the Portuguese autochthonous breeds of horses classified as threatened by the European Union. For this reason, it is essential to preserve this Animal Genetic Resources (AnGR), from the point of view of biodiversity and economic value. The identification of genetically superior horses for certain traits is important for the genetic evaluation of genetic improvement and conservation programs. In this context, the present work aimed to analyze the genotypes of horses of the Garrana breed in some genetic markers associated with pathologies and coat traits. In order to investigate the genotype of the 109 Garrana horses under study, DNA extraction was performed using the High Salt Method (Montegomery & Sise, 1990). Genotyping-by-sequencing (GBS) was performed using the AgriSeq Custom Panel - Equine 500 panel – Parentage & TD by Thermo Fisher Scientific, which allows knowing the genotype of horses in 500 SNP distributed in different genes. In this work, only the results of 37 of the 500 SNP were presented, of which 18 are linked to the definition of the equine coat and the remaining 19 are associated with resistance/susceptibility to diseases and the conformation of the animals. The horses did not show polymorphism among themselves for each SNP. Regarding the SNP associated with the definition of coats, the analysed horses did not present any alternative allele, so they should not express the correlated phenotypic traits. Regarding the SNP linked to resistance/susceptibility to diseases and the conformation of the animals, none of the horses revealed to be carriers of alternative alleles, thus not being able to transmit to their offspring the allele associated with the reported diseases.
The Garrana breed is one of the Portuguese autochthonous breeds of horses classified as threatened by the European Union. For this reason, it is essential to preserve this Animal Genetic Resources (AnGR), from the point of view of biodiversity and economic value. The identification of genetically superior horses for certain traits is important for the genetic evaluation of genetic improvement and conservation programs. In this context, the present work aimed to analyze the genotypes of horses of the Garrana breed in some genetic markers associated with pathologies and coat traits. In order to investigate the genotype of the 109 Garrana horses under study, DNA extraction was performed using the High Salt Method (Montegomery & Sise, 1990). Genotyping-by-sequencing (GBS) was performed using the AgriSeq Custom Panel - Equine 500 panel – Parentage & TD by Thermo Fisher Scientific, which allows knowing the genotype of horses in 500 SNP distributed in different genes. In this work, only the results of 37 of the 500 SNP were presented, of which 18 are linked to the definition of the equine coat and the remaining 19 are associated with resistance/susceptibility to diseases and the conformation of the animals. The horses did not show polymorphism among themselves for each SNP. Regarding the SNP associated with the definition of coats, the analysed horses did not present any alternative allele, so they should not express the correlated phenotypic traits. Regarding the SNP linked to resistance/susceptibility to diseases and the conformation of the animals, none of the horses revealed to be carriers of alternative alleles, thus not being able to transmit to their offspring the allele associated with the reported diseases.
Descrição
Mestrado em Engenharia Zootécnica - Produção Animal. Universidade de Lisboa, Instituto Superior de Agronomia, Faculdade de Medicina Veterinária
Palavras-chave
Garrana SNP genotyping-by-sequencing recursos genéticos animais fenótipo animal genetic resources phenotype
Contexto Educativo
Citação
Seixas, D.A.S.R. Estudo de polimorfismos associados ao tipo de pelagem e doenças na raça Garrana. Lisboa: ISA-Universidade de Lisboa, 2023. Dissertação de Mestrado
Editora
Instituto Superior de Agronomia, Universidade de Lisboa