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Molecular markers distinguishing T Cell subtypes with TSDR strand-bias methylation

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dc.contributor.authorMinskaia, Ekaterina
dc.contributor.authorSaraiva, Bárbara C.
dc.contributor.authorSoares, Maria Vieira
dc.contributor.authorAzevedo, Rita I.
dc.contributor.authorRibeiro, Ruy M.
dc.contributor.authorKumar, Saumya
dc.contributor.authorVieira, Ana I. S.
dc.contributor.authorLacerda, João
dc.date.accessioned2020-12-02T13:59:54Z
dc.date.available2020-12-02T13:59:54Z
dc.date.issued2018
dc.descriptionCopyright © 2018 Minskaia, Saraiva, Soares, Azevedo, Ribeiro, Kumar, Vieira and Lacerda. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.pt_PT
dc.description.abstractHuman regulatory CD4+CD25+FOXP3+ T cells (Treg) play important roles in the maintenance of self-tolerance and immune homeostasis in various disease settings and are also involved in the suppression of effective immune responses. These cells are heterogeneous in phenotype and function, and the ability to reliably distinguish between various FOXP3-expressing subpopulations can affect the development of successful therapies. This study demonstrates that hypomethylated CpG sites, present in four regions of the FOXP3 locus, CAMTA1 and FUT7 gene regions, can be used to distinguish several subsets of Treg from conventional CD4+ T lymphocytes (Tcon) in donors of both genders. We describe a previously unreported strand-bias hemimethylation pattern in FOXP3 promoter and TSDR in donors of both genders, with the coding strand being demethylated within promoter and methylated within TSDR in all CD4+ lymphocyte subtypes, whereas the template strand follows the previously described pattern of methylation with both regions being more demethylated in Treg subtypes and mostly methylated in Tcon. This strand-specific approach within the TSDR may prove to be instrumental in correctly defining Treg subsets in health and in disease.pt_PT
dc.description.sponsorshipThis research was funded by: Fundação para a Ciência e Tecnologia, Portugal under the Harvard Medical School–Portugal Program project Induction of Immune Tolerance in Human Allogeneic Hematopoietic Stem Cell Transplantation (HMSP-ICT/0001/2011), Gabinete de Apoio à Investigação Científica, Tecnológica e Inovação, GAPIC (Faculdade de Medicina ULisboa, GAPIC-04-2016 Project 20160011), LISBOA-01-0145-FEDER-007391 (project co-funded by FEDER through POR Lisboa 2020—Programa Operacional Regional de Lisboa, PORTUGAL 2020 and Fundação para a Ciência e a Tecnologia) and ENLIGHT-TEN project (European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement 675395).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFront Immunol. 2018 Nov 5; 9: 2540.pt_PT
dc.identifier.doi10.3389/fimmu.2018.02540pt_PT
dc.identifier.eissn1664-3224
dc.identifier.urihttp://hdl.handle.net/10451/45068
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontierspt_PT
dc.relationLISBOA-01-0145-FEDER-007391pt_PT
dc.relation.publisherversionhttps://www.frontiersin.org/journals/immunology#pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectStrand-bias methylationpt_PT
dc.subjectCAMTApt_PT
dc.subjectFOXP3pt_PT
dc.subjectFUT7pt_PT
dc.subjectEpigeneticspt_PT
dc.subjectRegulatory T lymphocytespt_PT
dc.titleMolecular markers distinguishing T Cell subtypes with TSDR strand-bias methylationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/HMSP-ICT%2F0001%2F2011/PT
oaire.citation.startPage2540pt_PT
oaire.citation.titleFrontiers in immunologypt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameMinskaia
person.familyNameGodinho A.V. D. Soares
person.familyNameAzevedo
person.familyNameRibeiro
person.familyNameKumar
person.familyNameVieira
person.familyNameLacerda
person.givenNameEkaterina
person.givenNameMaria
person.givenNameRita
person.givenNameRuy Miguel
person.givenNameSaumya
person.givenNameAna
person.givenNameJoão
person.identifier564538
person.identifier559297
person.identifier.ciencia-idF019-DCC5-CBF0
person.identifier.ciencia-id1512-E3F9-5FD4
person.identifier.ciencia-id9A15-E40B-738F
person.identifier.ciencia-idD511-03C9-4CF3
person.identifier.ciencia-idC817-9D62-CAEF
person.identifier.ciencia-id6412-2B63-2364
person.identifier.orcid0000-0002-1137-373X
person.identifier.orcid0000-0002-6492-2931
person.identifier.orcid0000-0002-5534-1481
person.identifier.orcid0000-0002-3988-8241
person.identifier.orcid0000-0001-9481-1944
person.identifier.orcid0000-0002-4045-3691
person.identifier.orcid0000-0003-1351-2809
person.identifier.ridI-3729-2013
person.identifier.scopus-author-id7203087102
person.identifier.scopus-author-id55954739900
person.identifier.scopus-author-id6603819609
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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