Publicação
Effect of oxidized LDL on erythrocyte nitric oxide metabolism
| dc.contributor.author | Napoleão, Patricia | |
| dc.contributor.author | Freitas, Teresa | |
| dc.contributor.author | Saldanha, Carlota | |
| dc.date.accessioned | 2018-07-17T11:41:18Z | |
| dc.date.available | 2018-07-17T11:41:18Z | |
| dc.date.issued | 2016 | |
| dc.description | © 2016 – IOS Press and the authors. All rights reserved | pt_PT |
| dc.description.abstract | AIMS: Oxidized low density lipoprotein (ox-LDL) has been reported as an inhibitor of nitric oxide (NO)-mediated dilatation in microcirculation. Oxidized LDL effect on NO metabolism of erythrocytes is not known. Therefore, this study aims to evaluate the effect of ox-LDL on erythrocytes NO metabolism. METHODS: The effect of different concentrations of human purified ox-LDL (25, 50 and 100 μg/mL) on NO metabolism was evaluated on blood of healthy subjects. RESULTS: An inhibitory effect of higher concentrations of ox-LDL on erythrocyte NO efflux levels was verified. Concentrations of NO efflux from erythrocytes were lower as consequence of treatments with 50 μg/mL ox-LDL treatment (1.6±0.27 nM) and 100 μg/mL ox-LDL treatment (1.3±0.22 nM) than control (1.9±0.28 nM). Opposite, ox-LDL incubation has a positive effect on GSNO content of erythrocytes. That effect is proportional to concentrations of ox-LDL treatments (10.8±1.4 nM for 25 μg/mL, 12.9±1.5 nM for 50 μg/mL and 12.1±1.9 nM for 100 μg/mL) and is significant relative to control (8.56±0.76 μM) and ACh (8.9±0.52 μM) aliquots. CONCLUSIONS: Presence of oxidized LDL in erythrocyte NO metabolism induces a decrease of NO efflux amount and an increase on intra-erythrocyte GSNO concentrations. These results suggest a role of ox-LDL in mobilization of NO between NO derivatives molecules in dependence of oxidized LDL concentration. An anti - reactive nitrogene role can be attributed to ox-LDL for its contribution in the erythrocyte scavenged ability for nitric oxide. | pt_PT |
| dc.description.sponsorship | Work was financially supported by Fundação para a Ciência e Tecnologia (SFRM/BPD/6308/2009). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Clinical Hemorheology and Microcirculation 64 (2016) 971–975 | pt_PT |
| dc.identifier.doi | 10.3233/CH-168025 | pt_PT |
| dc.identifier.issn | 1386-0291 | |
| dc.identifier.uri | http://hdl.handle.net/10451/34190 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | IOS Press | pt_PT |
| dc.relation | SFRM/BPD/6308/2009 | pt_PT |
| dc.relation.publisherversion | https://www.iospress.nl/journal/clinical-hemorheology-and-microcirculation/ | pt_PT |
| dc.subject | Oxidized LDL | pt_PT |
| dc.subject | Erythrocyte | pt_PT |
| dc.subject | Nitric oxide | pt_PT |
| dc.subject | S-nitrosoglutathione | pt_PT |
| dc.title | Effect of oxidized LDL on erythrocyte nitric oxide metabolism | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 975 | pt_PT |
| oaire.citation.issue | 4 | pt_PT |
| oaire.citation.startPage | 971 | pt_PT |
| oaire.citation.title | Clinical Hemorheology and Microcirculation | pt_PT |
| oaire.citation.volume | 64 | pt_PT |
| person.familyName | Napoleão | |
| person.givenName | Patrícia | |
| person.identifier.ciencia-id | C11E-8BE9-A97F | |
| person.identifier.orcid | 0000-0002-5896-1440 | |
| person.identifier.scopus-author-id | 8872775500 | |
| rcaap.rights | restrictedAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | c74643c7-434c-4310-9b98-2fcaa9fdd4b3 | |
| relation.isAuthorOfPublication.latestForDiscovery | c74643c7-434c-4310-9b98-2fcaa9fdd4b3 |