Vitis vinifera sex genes: from the wild to the domesticated varieties

Coito, João Lucas Fidalgo Oliveira

Publicação

Vitis vinifera sex genes: from the wild to the domesticated varieties

2018Tese de doutoramento

dc.contributor.advisor	Rocheta, Margarida
dc.contributor.advisor	Amâncio, Sara Barros Queiróz
dc.contributor.author	Coito, João Lucas Fidalgo Oliveira
dc.date.accessioned	2018-03-12T10:49:29Z
dc.date.available	2020-03-12T01:30:09Z
dc.date.issued	2018
dc.description	Doutoramento em Biologia / Instituto Superior de Agronomia	pt_PT
dc.description.abstract	Vitis vinifera vinifera is a domesticated hermaphrodite plant subspecies, and one of the most important crops in the world, required for wine making among other products. Vitis vinifera sylvestris is a dioecious subspecies considered the wild ancestral of V. v. vinifera. The molecular mechanisms responsible for the shift between hermaphroditism to dioecy are largely unknown. Male flowers of V. v. sylvestris show a reduced non-functional pistil while female plants exhibit reflexed stamens with infertile pollen. Hermaphrodite flowers develop functional male and female organs. In this work, the transcriptome of the three flower types were sequenced, assembled and de-novo assembled in order to map specific V. v. sylvestris transcripts, therefore understanding the primary players and mechanisms which contribute to the shift in flower types. This approach allowed for the detection of specific transcripts that might be responsible for the arrest of sexual organs in V. v. sylvestris flower type. Additionally, through transcription levels and RNA location, it seems that the ABCDE model genes are not responsible for the shift from hermaphroditism to dioecism and therefore sex determination mechanism most likely occurs downstream of the onset of flowering genes. The chromosome 2 was also specifically screened since it could contain a putative sex locus responsible for plant flower type determination. This analysis resulted in the development of two marker genes able to early differentiate between flower types, one of those genes specifically expressed only in the male flower carpels. Several crosses between both Vitis subspecies showed that the gene markers can predict the segregation based on flower type. Also, the genomic region where these genetic markers are located seem to be responsible for flower determination. It was possible to observe, that for VviAPRT3 and VviFSEX male plants are heterozygous dominant while female plants are homozygous recessive	pt_PT
dc.description.version	N/A	pt_PT
dc.identifier.citation	Coito, J.L.F.O. - Vitis vinifera sex genes: from the wild to the domesticated varieties. Lisboa: ISA, 2018, 291 p.	pt_PT
dc.identifier.tid	101560893
dc.identifier.uri	http://hdl.handle.net/10400.5/15072
dc.language.iso	eng	pt_PT
dc.publisher	ISA/UL	pt_PT
dc.relation	SFRH/BD/855824/2012	pt_PT
dc.subject	Vitis	pt_PT
dc.subject	dioecy	pt_PT
dc.subject	hermaphroditism	pt_PT
dc.subject	markers	pt_PT
dc.subject	development	pt_PT
dc.title	Vitis vinifera sex genes: from the wild to the domesticated varieties	pt_PT
dc.type	doctoral thesis
dspace.entity.type	Publication
oaire.awardNumber	PTDC/AGR-GPL/119298/2010
oaire.awardURI	info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-GPL%2F119298%2F2010/PT
oaire.fundingStream	3599-PPCDT
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.embargofct	No conteúdo da tese estão dados não publicados	pt_PT
rcaap.rights	openAccess	pt_PT
rcaap.type	doctoralThesis	pt_PT
relation.isProjectOfPublication	640ca651-f53b-47e6-bf54-2b64e8ae01ce
relation.isProjectOfPublication.latestForDiscovery	640ca651-f53b-47e6-bf54-2b64e8ae01ce