Logo do repositório
 
Publicação

Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases

2022-06Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorCosta, Monica
dc.contributor.authorPio, Luís Bernardo
dc.contributor.authorBule, Pedro
dc.contributor.authorDuarte, Marlene
dc.contributor.authorAlfaia, Cristina
dc.contributor.authorCoelho, Diogo
dc.contributor.authorBras, Joana
dc.contributor.authorFontes, Carlos M.G.A.
dc.contributor.authorPrates, José A.M
dc.contributor.authorCardoso, Vânia
dc.date.accessioned2022-07-07T17:10:33Z
dc.date.available2022-07-07T17:10:33Z
dc.date.issued2022-06
dc.descriptionResearch Areas: Agriculture; Veterinary Sciencespt_PT
dc.description.abstractABSTRACT - Green macroalgae, e.g., Ulva lactuca, are valuable bioactive sources of nutrients; but algae recalcitrant cell walls, composed of a complex cross-linked matrix of polysaccharides, can compromise their utilization as feedstuffs for monogastric animals. This study aimed to evaluate the ability of pre-selected Carbohy- drate-Active enZymes (CAZymes) and sulfatases to degrade U. lactuca cell walls and release nutritive compounds. A databank of 199 recombinant CAZymes and sulfatases was tested in vitro for their action towards U. lactuca cell wall polysaccharides. The enzymes were incubated with the macroalga, either alone or in combination, to release reducing sugars and decrease fluorescence intensity of Calcofluor White stained cell walls. The individual action of a polysaccharide lyase family 25 (PL25), an ulvan lyase, was shown to be the most efficient in cell wall disruption. The ulvan lyase treatment, in triplicate measures, promoted the release of 4.54 g/L (P < 0.001) reducing sugars, a mono- and oligosaccharides release of 11.4 and 11.2 mmol/100 g of dried alga (P < 0.01), respectively, and a decrease of 41.7% (P < 0.001) in cell wall fluorescence, in comparison to control. The ability of ulvan lyase treatment to promote the release of nutritional compounds from alga biomass was also evaluated. A release of some monounsaturated fatty acids was observed, particularly the health beneficial 18:1c9 (P < 0.001). How- ever, no significant release of total fatty acids (P > 0.05), proteins (P ¼ 0.861) or pigments (P > 0.05) was found. These results highlight the capacity of a single recombinant ulvan lyase (PL25 family) to incompletely disrupt U. lactuca cell walls. This enzyme could enhance the bioaccessibility of U. lactuca bioactive products with promising utilization in the feed industry.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta MM, Pio LB, Bule P, Cardoso VA, Duarte M, Alfaia CM, Coelho DF, Bras JA, Fontes CMGA, Prates JAM . 2022. Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases. Animal Nutrition, 9:p.184-192. DOI: https://doi.org/10.1016/j.aninu.2022.01.004pt_PT
dc.identifier.doihttps://doi.org/10.1016/j.aninu.2022.01.004pt_PT
dc.identifier.eissn2405-6545
dc.identifier.issn2405-6383
dc.identifier.urihttp://hdl.handle.net/10400.5/24781
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherKEAI PUBLISHINGpt_PT
dc.relationDisclosing the potential of seaweeds for feeding pigs and poultry
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationImproving the nutritional value of microalgae for feeding pigs through the use of novel enzymes
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2405654522000208?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMacroalgapt_PT
dc.subjectUlva lactucapt_PT
dc.subjectCarbohydrate-active enzymept_PT
dc.subjectUlvan lyasept_PT
dc.subjectCell wall degradationpt_PT
dc.titleRecalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyasespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDisclosing the potential of seaweeds for feeding pigs and poultry
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleImproving the nutritional value of microalgae for feeding pigs through the use of novel enzymes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FCAL-ZOO%2F30238%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F126198%2F2016/PT
oaire.citation.conferencePlacePEOPLES R. CHINApt_PT
oaire.citation.endPage192pt_PT
oaire.citation.startPage184pt_PT
oaire.citation.titleAnimal Nutritionpt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePereira Ribeiro Pio
person.familyNameBule
person.familyNameDuarte Santos
person.familyNameAlfaia
person.familyNameCoelho
person.familyNameFontes
person.familyNameMestre Prates
person.familyNameCardoso Lopes
person.givenNameLuís Bernardo
person.givenNamePedro
person.givenNameMarlene Rafaela Cardoso
person.givenNameCristina Maria Riscado Pereira Mateus
person.givenNameDiogo
person.givenNameCarlos
person.givenNameJosé António
person.givenNameVânia Alexandra da Silva
person.identifier6506672592
person.identifier.ciencia-id7017-5EE9-A60A
person.identifier.ciencia-id171E-F0CF-89F7
person.identifier.ciencia-idF712-6243-6678
person.identifier.ciencia-id7C16-D8D4-40C8
person.identifier.ciencia-id6A15-C6E1-71A0
person.identifier.ciencia-idC01A-FCB3-7F99
person.identifier.ciencia-id2617-53FF-04CD
person.identifier.ciencia-id121B-B572-2236
person.identifier.orcid0000-0003-2531-9926
person.identifier.orcid0000-0001-5634-699X
person.identifier.orcid0000-0001-8648-2204
person.identifier.orcid0000-0001-6151-8186
person.identifier.orcid0000-0002-1219-9753
person.identifier.orcid0000-0003-1032-5987
person.identifier.orcid0000-0001-8389-7543
person.identifier.ridK-9934-2013
person.identifier.scopus-author-id7005517465
person.identifier.scopus-author-id56003169500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8c7abc0-e136-4bbb-8427-5ed4b6f2d292
relation.isAuthorOfPublicatione3b85fbf-9c8b-49a4-8a8a-4b17c1820b9f
relation.isAuthorOfPublicationa22369ae-6c5e-4898-9c19-7337e2ca5acc
relation.isAuthorOfPublication7476f8a8-8730-4275-be88-3ada9510e66e
relation.isAuthorOfPublication5d00ebe9-f631-418e-8929-e97cde75018d
relation.isAuthorOfPublicationefb2611f-c4fd-4954-b932-653ae9792f2c
relation.isAuthorOfPublicationc5489c57-c896-4036-8b8a-6a4192846260
relation.isAuthorOfPublication36b1e43a-c5eb-4aae-91d9-20145f62532c
relation.isAuthorOfPublication.latestForDiscovery5d00ebe9-f631-418e-8929-e97cde75018d
relation.isProjectOfPublicationd0444e9a-40d1-417e-9da2-f7c71c2e1bef
relation.isProjectOfPublication8563a044-0c86-49a8-a275-8b1bb62cc0d8
relation.isProjectOfPublicationebf1a0f6-fc3a-4397-bfc4-1f70db436ed0
relation.isProjectOfPublication.latestForDiscoveryebf1a0f6-fc3a-4397-bfc4-1f70db436ed0

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases.pdf
Tamanho:
6.39 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CIISA - Artigos em revistas internacionais