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The open pan-genome architecture and virulence landscape of Mycobacterium bovis

2021-10Artigo científico Acesso aberto
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dc.contributor.authorReis, Ana C.
dc.contributor.authorCunha, Mónica V.
dc.date.accessioned2022-02-28T13:24:07Z
dc.date.available2022-02-28T13:24:07Z
dc.date.issued2021-10
dc.description.abstractAnimal tuberculosis (TB) is an emergent disease caused by Mycobacterium bovis, one of the animal-adapted ecotypes of the Mycobacterium tuberculosis complex (MTC). In this work, whole-genome comparative analyses of 70 M. bovis were performed to gain insights into the pan-genome architecture. The comparison across M. bovis predicted genome composition enabled clustering into the core- and accessory-genome components, with 2736 CDS for the former, while the accessory moiety included 3897 CDS, of which 2656 are restricted to one/two genomes only. These analyses predicted an open pan-genome architecture, with an average of 32 CDS added by each genome and show the diversification of discrete M. bovis subpopulations supported by both core- and accessory-genome components. The functional annotation of the pan-genome classified each CDS into one or several COG (Clusters of Orthologous Groups) categories, revealing ‘transcription’ (total average CDSs, n=258), ‘lipid metabolism and transport’ (n=242), ‘energy production and conversion’ (n=214) and ‘unknown function’ (n=876) as the most represented. The closer analysis of polymorphisms in virulence-related genes in a restrict group of M. bovis from a multi-host system enabled the identification of clade-monomorphic non-synonymous SNPs, illustrating clade-specific virulence landscapes and correlating with disease severity. This first comparative pan-genome study of a diverse collection of M. bovis encompassing all clonal complexes indicates a high percentage of accessory genes and denotes an open, dynamic non-conservative pan-genome structure, with high evolutionary potential, defying the canons of MTC biology. Furthermore, it shows that M. bovis can shape its virulence repertoire, either by acquisition and loss of genes or by SNP-based diversification, likely towards host immune evasion, adaptation and persistence.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationReis AC, Cunha MV. The open pan-genome architecture and virulence landscape of Mycobacterium bovis. Microb Genom. 2021;7(10):000664. doi:10.1099/mgen.0.000664pt_PT
dc.identifier.doi10.1099/mgen.0.000664pt_PT
dc.identifier.urihttp://hdl.handle.net/10451/51532
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMicrobiology Societypt_PT
dc.relationColossus: Control Of tuber cuLOsiS at the wildlife/livestock interface uSing innovative natUre based Solutions' (ref. POCI-01-0145- FEDER- 029783)pt_PT
dc.relationFCT UIDB/00329/2020pt_PT
dc.relationFCT UIDB/04046/2020pt_PT
dc.relationSystems epidemiology to devise new interventions for multi-host tuberculosis
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.titleThe open pan-genome architecture and virulence landscape of Mycobacterium bovispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPD/BD/128031/2016
oaire.awardTitleSystems epidemiology to devise new interventions for multi-host tuberculosis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/PD%2FBD%2F128031%2F2016/PT
oaire.citation.issue10pt_PT
oaire.citation.titleMicrobial Genomicspt_PT
oaire.citation.volume7pt_PT
oaire.fundingStreamFARH
person.familyNameCunha
person.givenNameMonica V.
person.identifier348477
person.identifier.ciencia-id5616-E715-2F31
person.identifier.orcid0000-0003-0401-0276
person.identifier.ridD-5544-2011
person.identifier.scopus-author-id7102711216
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa1b365bb-7421-4bf5-9cde-62b74c170e69
relation.isAuthorOfPublication.latestForDiscoverya1b365bb-7421-4bf5-9cde-62b74c170e69
relation.isProjectOfPublication3a390b9c-1b71-4b94-85cd-aa84b0047011
relation.isProjectOfPublication.latestForDiscovery3a390b9c-1b71-4b94-85cd-aa84b0047011

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