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Simple method for establishing primary Leporidae skin fibroblast cultures

2021-08-16Journal article Open access
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dc.contributor.authorAbade dos Santos, F.A.
dc.contributor.authorCarvalho, C. L.
dc.contributor.authorAlmeida, Isabel
dc.contributor.authorFAGULHA, TERESA
dc.contributor.authorRamos, Fernanda
dc.contributor.authorBarros, Sílvia C.
dc.contributor.authorHenriques, Margarida
dc.contributor.authorLuís, Tiago
dc.contributor.authorDuarte, Margarida D.
dc.date.accessioned2021-11-01T00:41:28Z
dc.date.available2021-11-01T00:41:28Z
dc.date.issued2021-08-16
dc.descriptionResearch Areas: Cell Biologypt_PT
dc.description.abstractCommercial hare and rabbit immortalized cell lines are extremely limited regarding the many species within the lagomorpha order. To overcome this limitation, researchers and technicians must establish primary cell cultures derived from biopsies or embryos. Among all cell types, fibroblasts are plastic and resilient cells, highly convenient for clinical and fundamental research but also for diagnosis, particularly for viral isolation. Here, we describe a fast and cheap method to produce primary fibroblast cell cultures from leporid species, using dispase II, a protease that allows dermal–epidermal separation, followed by a simple enzymatic digestion with trypsin. This method allows for the establishment of an in vitro cell culture system with an excellent viability yield and purity level higher than 85% and enables the maintenance and even immortalization of leporid fibroblastic cells derived from tissues already differentiated.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAbade dos Santos FA, Carvalho CL, Almeida I, Fagulha T, Ramos F, Barros SC, Henriques M, Luís T, Duarte MD. 2021. Simple method for establishing primary Leporidae skin fibroblast cultures. Cells 10(8). doi.org/10.3390/cells10082100pt_PT
dc.identifier.doi10.3390/cells10082100pt_PT
dc.identifier.eissn2073-4409
dc.identifier.urihttp://hdl.handle.net/10400.5/22455
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttp://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=11&SID=C2r7z1MmsTd9EJrylUm&page=1&doc=11pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPrimary fibroblastspt_PT
dc.subjectPrimary cell culturept_PT
dc.subjectVirus isolationpt_PT
dc.subjectMethodpt_PT
dc.subjectDispase IIpt_PT
dc.subjectLeporidaept_PT
dc.titleSimple method for establishing primary Leporidae skin fibroblast culturespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/71391/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157379/PT
oaire.citation.issue2100pt_PT
oaire.citation.titleCellspt_PT
oaire.citation.volumevol. 10(8)pt_PT
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAbade dos Santos
person.familyNameAlmeida
person.familyNameMARTINS FAGULHA
person.givenNameFábio
person.givenNameIsabel
person.givenNameTERESA MARIA
person.identifier.ciencia-idD810-0FEC-8051
person.identifier.ciencia-id7014-5DE5-07D9
person.identifier.orcid0000-0002-0696-7322
person.identifier.orcid0000-0002-2579-2337
person.identifier.orcid0000-0002-6509-6651
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationd911b01f-5425-46ae-8b02-dba40a22136b
relation.isAuthorOfPublication21aee4c7-c219-40ec-aa97-1401eae91d25
relation.isAuthorOfPublication.latestForDiscoveryacec0d1a-7e06-43bb-9140-94ae67608b0f
relation.isProjectOfPublication05a5082c-3078-416f-953f-34411997efa3
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