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Lineage-specific changes in mitochondrial properties during neural stem cell differentiation

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dc.contributor.authorSoares, Rita
dc.contributor.authorLourenço, Diogo M.
dc.contributor.authorF. Mota, Isa
dc.contributor.authorSebastião, Ana M
dc.contributor.authorXapelli, Sara
dc.contributor.authorMorais, Vanessa A.
dc.date.accessioned2024-05-02T13:13:36Z
dc.date.available2024-05-02T13:13:36Z
dc.date.issued2024
dc.description© 2024 Soares et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractNeural stem cells (NSCs) reside in discrete regions of the adult mammalian brain where they can differentiate into neurons, astrocytes, and oligodendrocytes. Several studies suggest that mitochondria have a major role in regulating NSC fate. Here, we evaluated mitochondrial properties throughout NSC differentiation and in lineage-specific cells. For this, we used the neurosphere assay model to isolate, expand, and differentiate mouse subventricular zone postnatal NSCs. We found that the levels of proteins involved in mitochondrial fusion (Mitofusin [Mfn] 1 and Mfn 2) increased, whereas proteins involved in fission (dynamin-related protein 1 [DRP1]) decreased along differentiation. Importantly, changes in mitochondrial dynamics correlated with distinct patterns of mitochondrial morphology in each lineage. Particularly, we found that the number of branched and unbranched mitochondria increased during astroglial and neuronal differentiation, whereas the area occupied by mitochondrial structures significantly reduced with oligodendrocyte maturation. In addition, comparing the three lineages, neurons revealed to be the most energetically flexible, whereas astrocytes presented the highest ATP content. Our work identified putative mitochondrial targets to enhance lineage-directed differentiation of mouse subventricular zone-derived NSCs.pt_PT
dc.description.sponsorshipThis work was supported by the European Molecular Biology Organization (EMBO), IG#3309; Fundação para a Ciência e Tecnologia (FCT) (PTDC/MED-NEU/7976/2020); International Society for Neurochemistry (ISN) Career Development Grant; and International Brain Research Organization (IBRO) Early Career Award. R Soares (PD/BD/128280/2017, COVID/BD/151619/2021, and IMM/BI/8-2021) and DM Lourenço (PD/BD/141784/2018 and COVID/BD/152658/2022) were in receipt of a fellowship from FCT. VA Morais is supported by FCT (IF/01693/2014; IMM/CT/27-2020). This project has received funding from H2020-WIDESPREAD-05-2020-Twinning (EpiEpinet) under grant agreement No 952455. We thank members of the VA Morais and AM Sebastião Labs for fruitful discussions. We would like to thank the BioImaging Facility, with a special thanks to José Rino, António Temudo, and Ana Nascimento and the Rodent Facility of Instituto de Medicina Molecular João Lobo Antunes for their technical support, and we also acknowledge the funding PPBI-POCI-01-0145-FEDER-022122.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLife Sci Alliance. 2024 Apr 25;7(7):e202302473pt_PT
dc.identifier.doi10.26508/lsa.202302473pt_PT
dc.identifier.eissn2575-1077
dc.identifier.urihttp://hdl.handle.net/10451/64635
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherLife Science Alliancept_PT
dc.relationPTDC/MED-NEU/7976/2020pt_PT
dc.relationPD/BD/128280/2017pt_PT
dc.relationCOVID/BD/151619/2021pt_PT
dc.relationIMM/BI/8-2021pt_PT
dc.relationPD/BD/141784/2018pt_PT
dc.relationCOVID/BD/152658/2022pt_PT
dc.relationIF/01693/2014pt_PT
dc.relationIMM/CT/27-2020pt_PT
dc.relation952455pt_PT
dc.relationPPBI-POCI-01-0145-FEDER-022122pt_PT
dc.relation.publisherversionhttps://www.life-science-alliance.org/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleLineage-specific changes in mitochondrial properties during neural stem cell differentiationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue7pt_PT
oaire.citation.titleLife Science Alliancept_PT
oaire.citation.volume7pt_PT
person.familyNameSoares
person.familyNameLourenço
person.familyNameF. Mota
person.familyNameSebastião
person.familyNameXapelli
person.familyNameMorais
person.givenNameRita
person.givenNameDiogo
person.givenNameIsa
person.givenNameAna M
person.givenNameSara
person.givenNameVanessa
person.identifier694579
person.identifier129383
person.identifier.ciencia-id6D10-6A4F-5B07
person.identifier.ciencia-id2512-BC8E-4984
person.identifier.ciencia-idF112-55E8-E37E
person.identifier.ciencia-id7110-C8C3-ED42
person.identifier.orcid0000-0003-2072-0358
person.identifier.orcid0000-0001-9994-0315
person.identifier.orcid0000-0002-6074-9916
person.identifier.orcid0000-0001-9030-6115
person.identifier.orcid0000-0001-6854-2509
person.identifier.orcid0000-0002-0830-0548
person.identifier.ridK-3959-2013
person.identifier.scopus-author-id7004409879
person.identifier.scopus-author-id8721560200
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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