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Dominance Between Plasmids Determines the Extent of Biofilm Formation

2020-08-26Artigo científico Acesso aberto
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dc.contributor.authorGama, João Alves
dc.contributor.authorFredheim, Elizabeth G. Aarag
dc.contributor.authorCléon, François
dc.contributor.authorReis, Ana Maria
dc.contributor.authorZilhão, Rita
dc.contributor.authorDionisio, Francisco
dc.date.accessioned2020-12-18T14:15:23Z
dc.date.available2020-12-18T14:15:23Z
dc.date.issued2020-08-26
dc.description.abstractBacterial biofilms have an impact in medical and industrial environments because they often confer protection to bacteria against harmful agents, and constitute a source from which microorganisms can disperse. Conjugative plasmids can enhance bacterial ability to form biofilms because conjugative pili act as adhesion factors. However, plasmids may interact with each other, either facilitating or inhibiting plasmid transfer. Accordingly, we asked whether effects on plasmid transfer also impacts biofilm formation. We measured biofilm formation of Escherichia coli cells harboring two plasmid types, or when the two plasmids were present in the same population but carried in different cells. Using eleven natural isolated conjugative plasmids, we confirmed that some indeed promote biofilm formation and, importantly, that this ability is correlated with conjugative efficiency. Further we studied the effect of plasmid pairs on biofilm formation. We observed increased biofilm formation in approximately half of the combinations when both plasmids inhabited the same cell or when the plasmids were carried in different cells. Moreover, in approximately half of the combinations, independent of the co-inhabitation conditions, one of the plasmids alone determined the extent of biofilm formation - thus having a dominant effect over the other plasmid. The molecular mechanisms responsible for these interactions were not evaluated here and future research is required to elucidate them.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGama JA, Fredheim EGA, Cléon F, Reis AM, Zilhão R and Dionisio F (2020) Dominance Between Plasmids Determines the Extent of Biofilm Formation. Front. Microbiol. 11:2070. doi: 10.3389/fmicb.2020.02070pt_PT
dc.identifier.doi10.3389/fmicb.2020.02070pt_PT
dc.identifier.urihttp://hdl.handle.net/10451/45453
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontierspt_PT
dc.relationJFCT grant SFRH/BD/86103/2012pt_PT
dc.relationUiT The Arctic University of Norway through UiT publication fund.pt_PT
dc.relationFCT UID/BIA/00329/2013 (cE3c)pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleDominance Between Plasmids Determines the Extent of Biofilm Formationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage2070pt_PT
oaire.citation.titleFrontiers in Microbiologypt_PT
oaire.citation.volume11pt_PT
person.familyNameDionísio
person.givenNameFrancisco
person.identifierA-2129-2009
person.identifier.ciencia-id7413-DB46-16E0
person.identifier.orcid0000-0002-3653-1511
person.identifier.scopus-author-id6506790127
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationaa2a19c2-e8bf-4b68-98f3-737d4515e13e
relation.isAuthorOfPublication.latestForDiscoveryaa2a19c2-e8bf-4b68-98f3-737d4515e13e

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