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Maximizing Blad-containing oligomerfungicidal activity in sweet cultivars of Lupinus albus seeds

2021Journal article Open access
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dc.contributor.authorCruz, Filipe
dc.contributor.authorBatista-Santos, Paula
dc.contributor.authorMonteiro, Sara
dc.contributor.authorNeves-Martins, J.
dc.contributor.authorFerreira, Ricardo Boavida
dc.date.accessioned2021-09-24T09:56:42Z
dc.date.available2021-09-24T09:56:42Z
dc.date.issued2021
dc.description.abstractDuring seed germination and plantlet growth, an important aspect of Lupinus β-conglutin proteolysis is the accumulation of blad in the cotyledons. Blad, a 20.4 kDa, 173 residue polypeptide which inhibits fungal growth, is the main subunit of BCO (Blad-containing oligomer), a Lupinus bioactive polypeptide oligomer, which underwent a successful translational research during the last thirty years. The development of this recent broad-spectrum biological fungicide for plant disease control made evident the advantage of being non-toxic to the environment, plants, humans and other animals, an interesting characteristic given the increasing consumer’s concern about food safety. The industrial-scale production of the edible fungicide BCO for agricultural purposes involves germinating and growing for ca. 8 days massive amounts of Lupinus plantlets. Therefore, it becomes economically relevant to maximize/increment the amount of extractable BCO. Although BCO represents an alternative pathway for lupin production in Europe, there is no previous evidence on lupin cultivars concerning BCO activity or its cotyledonary concentration, and therefore on the most promising cultivars for BCO extraction 16 and fungicide production. In this work, the amount of BCO and its level of fungicide activity was evaluated in seven sweet cultivars of L. albus (cvs. Amiga, Energy, Estoril, Ludic, Misak, Multitalia and Rumbo), along 20 days after the onset of germination. In addition, four distinct lots of cv. Energy and six lots of cv Misak, with different harvest years (1997, 2010, 2013 and 2014) and/or different sites of production in Portugal (Alto Alentejo, Baixo Alentejo and Beira Litoral) were also analysed. Quantitative assays demonstrated maximal accumulation of BCO in the cotyledons of 4-days-old L. albus plantlets, apart from significant differences between seeds’ harvest year or production site. The assays also showed that cv. Energy had significant higher accumulation of BCO (31.67 μg mg-1 wet weight) than Rumbo (25.67 μg mg-1) and Misak (22.12 μg mg-1), respectively second and third cultivars with highest accumulation of the oligomer. Additionally, very significant differences were also observed among seeds’ harvest year or production site. These observations reduced dramatically the original purpose of this work on the study of BCO variations at the level of sweet L. albus cultivars, since the ‘noise’ detected in BCO levels caused by the year and location of their production was far greater than its variation among cultivars. The observation that storing for several years the seeds at -20ºC did not seem to decrease BCO activity and suggests that the variation in cotyledonary BCO accumulation is essentially controlled by the prevailing edaphoclimatic conditions during seed formation. Furthermore, the activity tests exhibited significant antifungal activity between 3 and 5 days-old plantlets, with absence of antifungal activity on 8 days-old plantlets or older. Overall this study is of considerable importance to maximize BCO extraction from the cotyledons of sweet L. albus cultivarspt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationCruz, Filipe; Batista-Santos, Paula; Monteiro, Sara; Neves-Martins, João; Ferreira, Ricardo Boavida. "Maximizing Blad-containing oligomer fungicidal activity in sweet cultivars of Lupinus albus seeds". Industrial Crops and Products 162 (2021): 113242pt_PT
dc.identifier.doihttp://dx.doi.org/10.1016/j.indcrop.2021.113242pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/21982
dc.language.isoengpt_PT
dc.relationUID/AGR/04129/2020pt_PT
dc.subjectBladpt_PT
dc.subjectBCOpt_PT
dc.subjectLupinus albuspt_PT
dc.subjectantifungal activitypt_PT
dc.subjectfungicide productionpt_PT
dc.subjectseed germinationpt_PT
dc.titleMaximizing Blad-containing oligomerfungicidal activity in sweet cultivars of Lupinus albus seedspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleIndustrial Crops and Productspt_PT
person.familyNameBento Batista dos Santos
person.familyNameNeves Martins
person.familyNameFerreira
person.givenNamePaula Cristina
person.givenNameJoão
person.givenNameRicardo Boavida
person.identifierX-9778-2018
person.identifier86030
person.identifier.ciencia-idED1E-E33A-0B5A
person.identifier.ciencia-id7E1A-3F69-EE24
person.identifier.ciencia-id371D-28A0-4027
person.identifier.orcid0000-0002-5442-1092
person.identifier.orcid0000-0002-1331-1081
person.identifier.orcid0000-0002-5027-7564
person.identifier.scopus-author-id36087137800
person.identifier.scopus-author-id7801484626
person.identifier.scopus-author-id8285312700
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication8b9c9a04-7672-4b02-9eec-de91cd2b79c7
relation.isAuthorOfPublication5b27dc8d-d2da-45a5-9902-d8ae6992936f
relation.isAuthorOfPublication0f32c437-6c87-47d7-998b-c97c4695806a
relation.isAuthorOfPublication.latestForDiscovery0f32c437-6c87-47d7-998b-c97c4695806a

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