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Molecular maps of synovial cells in inflammatory arthritis using an optimized synovial tissue dissociation protocol

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dc.contributor.authorEdalat, Sam G.
dc.contributor.authorGerber, Reto
dc.contributor.authorHoutman, Miranda
dc.contributor.authorLückgen, Janine
dc.contributor.authorTeixeira, Rui Lourenço
dc.contributor.authorPalacios Cisneros, Maria del Pilar
dc.contributor.authorPfanner, Tamara
dc.contributor.authorKuret, Tadeja
dc.contributor.authorIžanc, Nadja
dc.contributor.authorMicheroli, Raphael
dc.contributor.authorPolido Pereira, Joaquim
dc.contributor.authorSaraiva, Fernando
dc.contributor.authorLingam, Swathi
dc.contributor.authorBurki, Kristina
dc.contributor.authorBurja, Blaž
dc.contributor.authorPauli, Chantal
dc.contributor.authorRotar, Žiga
dc.contributor.authorTomšič, Matija
dc.contributor.authorČučnik, Saša
dc.contributor.authorFonseca, João Eurico
dc.contributor.authorDistler, Oliver
dc.contributor.authorCalado, Ângelo
dc.contributor.authorRomão, Vasco C.
dc.contributor.authorOspelt, Caroline
dc.contributor.authorSodin-Semrl, Snežna
dc.contributor.authorRobinson, Mark D.
dc.contributor.authorFrank Bertoncelj, Mojca
dc.date.accessioned2024-07-23T10:44:25Z
dc.date.available2024-07-23T10:44:25Z
dc.date.issued2024
dc.description© 2024 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).pt_PT
dc.description.abstractIn this study, we optimized the dissociation of synovial tissue biopsies for single-cell omics studies and created a single-cell atlas of human synovium in inflammatory arthritis. The optimized protocol allowed consistent isolation of highly viable cells from tiny fresh synovial biopsies, minimizing the synovial biopsy drop-out rate. The synovium scRNA-seq atlas contained over 100,000 unsorted synovial cells from 25 synovial tissues affected by inflammatory arthritis, including 16 structural, 11 lymphoid, and 15 myeloid cell clusters. This synovial cell map expanded the diversity of synovial cell types/states, detected synovial neutrophils, and broadened synovial endothelial cell classification. We revealed tissue-resident macrophage subsets with proposed matrix-sensing (FOLR2+COLEC12high) and iron-recycling (LYVE1+SLC40A1+) activities and identified fibroblast subsets with proposed functions in cartilage breakdown (SOD2highSAA1+SAA2+SDC4+) and extracellular matrix remodeling (SERPINE1+COL5A3+LOXL2+). Our study offers an efficient synovium dissociation method and a reference scRNA-seq resource, that advances the current understanding of synovial cell heterogeneity in inflammatory arthritis.pt_PT
dc.description.sponsorshipThis study was funded by research grants from the Vontobel Foundation, OPO-Foundation, Novartis Foundation for Biomedical Research, SNSF Research Grant No. 10030_204869, SNSF Research Grant No. 176061, research grants from Marie-Lou Ringgenberg Foundation, Stiftung für Rheumaforschung, Swiss Rheumatology Society and medAlumni, University of Zurich.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationiScience. 2024 Apr 10;27(6):109707pt_PT
dc.identifier.doi10.1016/j.isci.2024.109707pt_PT
dc.identifier.eissn2589-0042
dc.identifier.urihttp://hdl.handle.net/10451/65405
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/isciencept_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHealth sciencespt_PT
dc.subjectOmicspt_PT
dc.subjectTechnical aspects of cell biologypt_PT
dc.subjectTranscriptomicspt_PT
dc.titleMolecular maps of synovial cells in inflammatory arthritis using an optimized synovial tissue dissociation protocolpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue6pt_PT
oaire.citation.titleiSciencept_PT
oaire.citation.volume27pt_PT
person.familyNameTeixeira
person.familyNamePolido Pereira
person.familyNameSaraiva
person.familyNameFonseca
person.familyNameCalado
person.familyNameC Romão
person.givenNameRui Lourenço
person.givenNameJoaquim
person.givenNameFernando
person.givenNameJoão
person.givenNameÂngelo
person.givenNameVasco
person.identifier676523
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person.identifier.ciencia-id6010-D535-75A2
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person.identifier.ciencia-idF310-B85D-57C7
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person.identifier.orcid0000-0003-1432-3671
person.identifier.orcid0000-0003-0182-2615
person.identifier.orcid0000-0002-5603-9436
person.identifier.ridY-2296-2019
person.identifier.ridO-8224-2014
person.identifier.scopus-author-id7101983519
person.identifier.scopus-author-id55989452300
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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