A carregar...
Publicação
Estudo da biodisponibilidade do DHA decorrente de uma alimentação rica em pescado: ensaio in vivo num modelo animal para a Doença de Alzheimer
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 1.28 MB | Adobe PDF |
Autores
Orientador(es)
Resumo(s)
A Doença de Alzheimer (DA) é uma doença neurodegenerativa caracterizada pela perda progressiva das
funções cognitivas. Na ausência de tratamento eficaz, a prevenção através da nutrição pode constituir
uma abordagem promissora. O ácido docosahexaenóico (DHA) é essencial para o cérebro e, a sua
deficiência, juntamente com alterações no metabolismo lipídico, foi associada à DA e ao declínio
cognitivo. Desconhecendo o impacto desta alteração noutros órgãos, este estudo teve como objetivo
avaliar a biodisponibilidade do DHA nos rins e testículos e perceber a sua influência no seu
funcionamento no contexto da DA. Assim, 32 murganhos transgénicos 5xFAD (modelo animal da DA)
foram divididos em quatro grupos e alimentados com dietas distintas: Controlo (C) (AIN-93M), Cavala
(CV) (AIN-93M com 10% cavala), Quinoa (Q) (AIN-93M com 5% quinoa), Cavala + Quinoa (CVQ)
(AIN-93M com 10% cavala e 5% quinoa). Caracterizou-se a fração lipídica destes tecidos através do
teor de lípidos totais, perfil de classes de lípidos, perfil de ácidos gordos e perfil de ácidos gordos nas
principais classes lipídicas. O teor de lípidos totais não foi afetado pelas dietas, porém, foi percetível um
aumento significativo no teor de DHA nos rins 20,6% e nos testículos 5,5% na dieta CVQ. Nas classes
de lípidos, houve mais diferenças significativas nos triacilgliceróis (TAG) nos rins e nos diacilgliceróis
nos testículos. Quanto ao perfil de ácidos gordos das principais classes lipídicas renais, as diferenças
observadas foram mais marcantes nos ácidos gordos livres (FFA) e TAG, com níveis superiores de DHA
no grupo CVQ (11,0% e 12,1%, respetivamente). Os resultados revelaram que a combinação de cavala
e quinoa proporcionou maior benefício, com a quinoa a potenciar o efeito da cavala, aumentando a
incorporação de DHA nos órgãos. Este estudo demonstrou que esta abordagem nutricional pode
contribuir para a melhoria da saúde renal e testicular em indivíduos com DA.
Alzheimer's disease (AD) is a neurodegenerative disease characterized by the progressive loss of cognitive functions. Without an effective treatment, nutrition may be a promising prevention approach. Docosahexaenoic acid (DHA) is essential for the brain and, its deficiency, together with changes in lipid metabolism, has been associated with AD and cognitive decline. Unknowing the impact of this alteration on other organs, this study aimed to assess the bioavailability of DHA in the kidneys and testes and to understand its influence on the functioning of these organs in the context of AD. Therefore, 32 5xFAD transgenic mice (animal model of AD) were divided into four groups and fed different diets: Control (C) (AIN-93M), Mackerel (CV) (AIN-93M with 10% mackerel), Quinoa (Q) (AIN-93M with 5% quinoa), Mackerel + Quinoa (CVQ) (AIN-93M with 10% mackerel and 5% quinoa). The lipid fraction of these tissues was characterized through total lipid content, lipid class profile, fatty acid profile and fatty acid profile in the main lipid classes. The total lipid content was not affected by the diets, but there was a significant increase in the DHA content in the kidney by 20,6% (CVQ) and in the testicle by 5,5% (CVQ). Among lipid classes, there were more significant differences in triacylglycerols (TAG) in the kidneys and diacylglycerols in the testes. Regarding the fatty acid profile of the main renal lipid classes, the differences were more marked in free fatty acids (FFA) and TAG, with higher levels of DHA in the CVQ group (11,0% and 12,1%, respectively). The results showed that the combination of mackerel and quinoa provided the greatest benefit, with the quinoa enhancing the effect of the mackerel by increasing the incorporation of DHA into the organs. This study showed that this nutritional approach can contribute to improving kidney and testicular health in individuals with AD.
Alzheimer's disease (AD) is a neurodegenerative disease characterized by the progressive loss of cognitive functions. Without an effective treatment, nutrition may be a promising prevention approach. Docosahexaenoic acid (DHA) is essential for the brain and, its deficiency, together with changes in lipid metabolism, has been associated with AD and cognitive decline. Unknowing the impact of this alteration on other organs, this study aimed to assess the bioavailability of DHA in the kidneys and testes and to understand its influence on the functioning of these organs in the context of AD. Therefore, 32 5xFAD transgenic mice (animal model of AD) were divided into four groups and fed different diets: Control (C) (AIN-93M), Mackerel (CV) (AIN-93M with 10% mackerel), Quinoa (Q) (AIN-93M with 5% quinoa), Mackerel + Quinoa (CVQ) (AIN-93M with 10% mackerel and 5% quinoa). The lipid fraction of these tissues was characterized through total lipid content, lipid class profile, fatty acid profile and fatty acid profile in the main lipid classes. The total lipid content was not affected by the diets, but there was a significant increase in the DHA content in the kidney by 20,6% (CVQ) and in the testicle by 5,5% (CVQ). Among lipid classes, there were more significant differences in triacylglycerols (TAG) in the kidneys and diacylglycerols in the testes. Regarding the fatty acid profile of the main renal lipid classes, the differences were more marked in free fatty acids (FFA) and TAG, with higher levels of DHA in the CVQ group (11,0% and 12,1%, respectively). The results showed that the combination of mackerel and quinoa provided the greatest benefit, with the quinoa enhancing the effect of the mackerel by increasing the incorporation of DHA into the organs. This study showed that this nutritional approach can contribute to improving kidney and testicular health in individuals with AD.
Descrição
Tese de mestrado, Biologia Humana e Ambiente, 2024, Universidade de Lisboa, Faculdade de Ciências
Palavras-chave
Nutrição envelhecimento n-3 PUFA saúde renal saúde testicular Teses de mestrado - 2024