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Transcriptional, hormonal, and metabolic changes in susceptible grape berries under powdery mildew infection

2021Journal article Open access
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dc.contributor.authorPimentel, Diana
dc.contributor.authorAmaro, Rute
dc.contributor.authorErban, Alexander
dc.contributor.authorMauri, Nuria
dc.contributor.authorSoares, Flávio
dc.contributor.authorRego, Cecília
dc.contributor.authorMartínez-Zapater, José M.
dc.contributor.authorMithofer, Axel
dc.contributor.authorKopka, Joachim
dc.contributor.authorFortes, Ana Margarida
dc.date.accessioned2021-09-23T15:40:55Z
dc.date.available2021-09-23T15:40:55Z
dc.date.issued2021
dc.description.abstractGrapevine (Vitis vinifera) berries are extremely sensitive to infection by the biotrophic pathogen Erysiphe necator, causing powdery mildew disease with deleterious effects on grape and wine quality. The combined analysis of the transcriptome and metabolome associated with this common fungal infection has not been previously carried out in any fruit. In order to identify the molecular, hormonal, and metabolic mechanisms associated with infection, healthy and naturally infected V. vinifera cv. Carignan berries were collected at two developmental stages: late green (EL33) and early véraison (EL35). RNA sequencing combined with GC–electron impact ionization time-of-flight MS, GC–electron impact ionization/quadrupole MS, and LC–tandem MS analyses revealed that powdery mildew-susceptible grape berries were able to activate defensive mechanisms with the involvement of salicylic acid and jasmonates and to accumulate defense-associated metabolites (e.g. phenylpropanoids, fatty acids). The defensive strategies also indicated organ-specific responses, namely the activation of fatty acid biosynthesis. However, defense responses were not enough to restrict fungal growth. The fungal metabolic program during infection involves secretion of effectors related to effector-triggered susceptibility, carbohydrate-active enzymes and activation of sugar, fatty acid, and nitrogen uptake, and could be under epigenetic regulation. This study also identified potential metabolic biomarkers such as gallic, eicosanoic, and docosanoic acids and resveratrol, which can be used to monitor early stages of infectionpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi.org/10.1093/jxb/erab258pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.5/21974
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSEBpt_PT
dc.relationPTDC/ASPHOR/ 28485/2017pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectbiotic stresspt_PT
dc.subjectErysiphe necatorpt_PT
dc.subjectgrapevinept_PT
dc.subjecthormonal profilingpt_PT
dc.subjectmetabolomept_PT
dc.subjectplant defensept_PT
dc.subjectpowdery mildewpt_PT
dc.subjecttranscriptomept_PT
dc.subjectsusceptibilitypt_PT
dc.subjectVitis viniferapt_PT
dc.titleTranscriptional, hormonal, and metabolic changes in susceptible grape berries under powdery mildew infectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157499/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157816/PT
oaire.citation.titleJournal of Experimental Botanypt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsEmbargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication4b3c6d5b-a649-475e-b92f-36045208e552
relation.isProjectOfPublication3c6b976b-d698-4b61-827d-f7fb2121bd14
relation.isProjectOfPublication.latestForDiscovery3c6b976b-d698-4b61-827d-f7fb2121bd14

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