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Transcriptome profiling of two Iberian freshwater fish exposed to thermal stress

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dc.contributor.authorJesus, Tiago
dc.contributor.authorGrosso, Ana Rita
dc.contributor.authorAlmeida-Val, Vera Maria Fonseca
dc.contributor.authorCoelho, Maria Manuela
dc.date.accessioned2022-06-20T15:38:03Z
dc.date.available2022-06-20T15:38:03Z
dc.date.issued2016
dc.description© 2015 Elsevier Ltd. All rights reserved.pt_PT
dc.description.abstractThe congeneric freshwater fish Squalius carolitertii and S. torgalensis inhabit different Iberian regions with distinct climates; Atlantic in the North and Mediterranean in the South, respectively. While northern regions present mild temperatures, fish in southern regions often experience harsh temperatures and droughts. Previous work with two hsp70 genes suggested that S. torgalensis is better adapted to harsher thermal conditions than S. carolitertii as a result of the different environmental conditions. We present a transcriptomic characterisation of these species' thermal stress responses. Through differential gene expression analysis of the recently available transcriptomes of these two endemic fish species, comprising 12 RNA-seq libraries from three tissues (skeletal muscle, liver and fins) of fish exposed to control (18 °C) and test (30 °C) conditions, we intend to lay the foundations for further studies on the effects of temperature given predicted climate changes. Results showed that S. carolitertii had more upregulated genes, many of which are involved in transcription regulation, whereas S. torgalensis had more downregulated genes, particularly those responsible for cell division and growth. However, both species displayed increased gene expression of many hsps genes, suggesting that they are able to deal with protein damage caused by heat, though with a greater response in S. torgalensis. Together, our results suggest that S. torgalensis may have an energy saving strategy during short periods of high temperatures, re-allocating resources from growth to stress response mechanisms. In contrast, S. carolitertii regulates its metabolism by increasing the expression of genes involved in transcription and promoting the stress response, probably to maintain homoeostasis. Additionally, we indicate a set of potential target genes for further studies that may be particularly suited to monitoring the responses of Cyprinidae to changing temperatures, particularly for species living in similar conditions in the Mediterranean Peninsulas.pt_PT
dc.description.sponsorshipThis work was supported by FCT Strategic project Pest-OE/BIA/UI0329/2014, FCT Research Fellowship SFRH/BD/73801/2010, CAPES/FCT No. 320/11, INCT-ADAPTA (FAPEAM/CNPq 573976/2008-2), and CNPq Research Fellowship 305744/2011-9.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Therm Biol. 2016 Jan;55:54-61pt_PT
dc.identifier.doi10.1016/j.jtherbio.2015.11.009pt_PT
dc.identifier.eissn1879-0992
dc.identifier.issn0306-4565
dc.identifier.urihttp://hdl.handle.net/10451/53413
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation2014 - Strategic Project
dc.relationADAPTATION OF FRESHWATER FISH TO OXYGEN AND TEMPERATURE STRESS – A MOLECULAR STUDY IN TWO ENVIRONMENTAL CONTEXTS
dc.relation.publisherversionhttps://www.sciencedirect.com/journal/journal-of-thermal-biologypt_PT
dc.subjectCyprinidaept_PT
dc.subjectGene expressionpt_PT
dc.subjectRNA-seqpt_PT
dc.subjectSqualiuspt_PT
dc.subjectTemperaturept_PT
dc.titleTranscriptome profiling of two Iberian freshwater fish exposed to thermal stresspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPEst-OE/BIA/UI0329/2014
oaire.awardNumberSFRH/BD/73801/2010
oaire.awardTitle2014 - Strategic Project
oaire.awardTitleADAPTATION OF FRESHWATER FISH TO OXYGEN AND TEMPERATURE STRESS – A MOLECULAR STUDY IN TWO ENVIRONMENTAL CONTEXTS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FBIA%2FUI0329%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F73801%2F2010/PT
oaire.citation.endPage61pt_PT
oaire.citation.startPage54pt_PT
oaire.citation.titleJournal of Thermal Biologypt_PT
oaire.citation.volume55pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNameJesus
person.familyNameGrosso
person.familyNameCoelho
person.givenNameTiago
person.givenNameAna Rita
person.givenNameMaria
person.identifierI-6656-2013
person.identifier.ciencia-id3D18-571E-133A
person.identifier.ciencia-id3A15-8A0A-6687
person.identifier.orcid0000-0001-8054-4136
person.identifier.orcid0000-0001-6974-4209
person.identifier.orcid0000-0003-3288-1693
person.identifier.ridB-8672-2009
person.identifier.scopus-author-id26639262500
person.identifier.scopus-author-id26643445100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication607e3b4e-024c-4fae-83fe-e0ed9c3f66a8
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