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Biofilms of Listeria monocytogenes produced at 12ÂșC either in pure culture or in co-culture with Pseudomonas aeruginosa showed reduced susceptibility to sanitizers
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Orientador(es)
Resumo(s)
The biofilm-forming ability of 21 Listeria monocytogenes isolates, previously pulsotyped and corresponding to
16 strains, from different origins was evaluated using the Calgary Biofilm DeviceR , at 37 âŠC. Biofilms of 4 selected
strains were also produced either on pure cultures or on co-cultures with Pseudomonas aeruginosa (PAO1), at 12 âŠC and at
37 âŠC. For these biofilms, the minimum biofilm eradication concentrations (MBECs) of 4 commercial dairy sanitizers (1
alkyl amine acetate basedâT99, 2 chlorine basedâT66 and DD, and 1 phosphoric acid basedâBP) were determined.
Listeria monocytogenes biofilms grown, either at 37 âŠC or 12 âŠC, were able to achieve similar cell densities by using different
incubation periods (24 h and 7 d, respectively). In co-culture biofilms, P. aeruginosa was the dominant species, either at
37 âŠC or at 12 âŠC, representing 99% of a total biofilm population of 6 to 7 log CFU/peg. Co-culture biofilms were
generally less susceptible than L. monocytogenes pure cultures. More interestingly, the biofilms produced at 12 âŠC were
usually less susceptible to the sanitizers than when produced at 37 âŠC. Single or co-culture biofilms of L. monocytogenes
and PAO1, particularly produced at 12 âŠC, retrieved MBEC values for agents T99 and BP that were, at times, above the
maximum in-use recommended concentrations for these agents. The results presented here reinforce the importance of
the temperature used for biofilm formation, when susceptibility to sanitizers is being assessed
Descrição
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Palavras-chave
biofilms Calgary biofilm device Listeria monocytogenes low temperature sanitizers
Contexto Educativo
Citação
"Journal of Food Science". ISSN 1750-3841. 76(2) (2011) M143-M148