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Depletion of the yeast nuclear exosome subunit Rrp6 results in accumulation of Polyadenylated RNAs in a discrete domain within the nucleolus

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dc.contributor.authorCarneiro, Tiago
dc.contributor.authorCarvalho, Célia
dc.contributor.authorBraga, José
dc.contributor.authorRino, José
dc.contributor.authorMilligan, Laura
dc.contributor.authorTollervey, David
dc.contributor.authorCarmo-Fonseca, Maria
dc.date.accessioned2021-05-14T11:45:04Z
dc.date.available2021-05-14T11:45:04Z
dc.date.issued2007
dc.descriptionCopyright © 2007, American Society for Microbiology. All Rights Reservedpt_PT
dc.description.abstractRecent data reveal that a substantial fraction of transcripts generated by RNA polymerases I, II, and III are rapidly degraded in the nucleus by the combined action of the exosome and a noncanonical poly(A) polymerase activity. This work identifies a domain within the yeast nucleolus that is enriched in polyadenylated RNAs in the absence of the nuclear exosome RNase Rrp6 or the exosome cofactor Mtr4. In normal yeast cells, poly(A)(+) RNA was undetectable in the nucleolus but the depletion of either Rrp6 or Mtr4 led to the accumulation of polyadenylated RNAs in a discrete subnucleolar region. This nucleolar poly(A) domain is enriched for the U14 snoRNA and the snoRNP protein Nop1 but is distinct from the nucleolar body that functions in snoRNA maturation. In strains lacking both Rrp6 and the poly(A) polymerase Trf4, the accumulation of poly(A)(+) RNA was suppressed, suggesting the involvement of the Trf4-Air1/2-Mtr4 polyadenylation (TRAMP) complex. The accumulation of polyadenylated snoRNAs in a discrete nucleolar domain may promote their recognition as substrates for the exosome.pt_PT
dc.description.sponsorshipT.C. was the recipient of a fellowship from Fundação para a Ciência e Tecnologia, Portugal. This work was supported by the European Commission QLG2-CT-2001-01554. D.T. and L.M. were supported by the Wellcome Trust.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMol Cell Biol. 2007 Jun;27(11):4157-4165pt_PT
dc.identifier.doi10.1128/MCB.00120-07pt_PT
dc.identifier.eissn1098-5549
dc.identifier.issn0270-7306
dc.identifier.urihttp://hdl.handle.net/10451/47857
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relationQLG2-CT-2001-01554pt_PT
dc.relation.publisherversionhttps://mcb.asm.org/pt_PT
dc.titleDepletion of the yeast nuclear exosome subunit Rrp6 results in accumulation of Polyadenylated RNAs in a discrete domain within the nucleoluspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage4165pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage4157pt_PT
oaire.citation.titleMolecular and Cellular Biologypt_PT
oaire.citation.volume27pt_PT
person.familyNameCarvalho
person.familyNameBraga
person.familyNameRino Henriques
person.familyNameCarmo-Fonseca
person.givenNameCélia
person.givenNameJosé
person.givenNameJosé Miguel
person.givenNameMaria
person.identifier.ciencia-idDD18-667D-59FE
person.identifier.ciencia-id621A-6802-47DB
person.identifier.ciencia-idA117-FBDB-A27B
person.identifier.ciencia-idB31F-0435-0753
person.identifier.orcid0000-0002-5980-595X
person.identifier.orcid0000-0002-2255-6486
person.identifier.orcid0000-0002-0637-4527
person.identifier.orcid0000-0002-3402-7143
person.identifier.scopus-author-id7007128195
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication0bc60872-4f9a-44d0-9bd6-13c82c3298c2
relation.isAuthorOfPublication38513804-5830-4012-b517-47aeb4938601
relation.isAuthorOfPublication54f3b487-a7a1-4597-aa2e-65f660855937
relation.isAuthorOfPublicationf4cae50c-2389-4b6a-8e3a-8fd8e538add0
relation.isAuthorOfPublication.latestForDiscovery54f3b487-a7a1-4597-aa2e-65f660855937

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